Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Identify the carbon source used for cellulase production and give TWO examples
of mesoentrient elements.
Transcribed Image Text:Medium used for cellulase production contained (g/L): NANO3, 3; MgSO4-7H2O, 0.5;
KHPO4, 1; KCI, 0.5 and supplemented with 1 mL FeSO5-7H2O as trace element. The
medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus
spore suspension (approximately 2x10' spores/mL) to 50 mL of sterilized medium
containing 5 % (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at
35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization
before proceeding to statistical optimization.
Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme
activity of Aspergillus terreus
Concentration
Endoglucanase
activity (U mL')
Protein content
Specific activity (U
mg' protein)
(mg mL-)
Bagasse (%)
1
0.32
0.90
0.35
0.47
1.83
0.25
3
0.65
2.50
0.26
4
0.88
3.16
0.28
5
0.96
3.30
0.29
0.73
1.88
0.39
Yeast extract (g L)
2.0
1.40
2.17
0.65
2.5
1.90
1.82
1.04
3.0
2.47
2.04
1.21
3.5
1.99
2.70
0.74
4.0
1.67
2.68
0.63
4.5
1.51
3.10
0.48
5.0
1.35
3.76
0.35
5.5
1.18
3.20
0.37
6.0
074
2.52
0.29
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