Identify and match the possible cause(s) of the following unexpected results in agarose gel electrophoresis. (Right minus wrong) Wrong polarity; melted gel; over-run electrophoresis; complete or partial degradation of DNA template. Defective primers [Choose] Entirely empty gel PCR failed, primarily due to missing/degraded template and/or Chelex resi Band appearing in the lane of positive control, but not others Missing band in the lane of positive control contamination. Band appearing in the lane of negative control Sample was contaminated and the contaminating DNA was amplified. [Choose] Template DNA extracted from food sample was degraded or contaminated with Chelex resin [Choose]

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Identify and match the possible cause(s) of the following unexpected results in agarose gel electrophoresis. (Right minus wrong)
Wrong polarity; melted gel; over-run electrophoresis; complete or partial
degradation of DNA template. Defective primers.
✓ [Choose ]
Entirely empty gel
PCR failed, primarily due to missing/degraded template and/or Chelex resi
contamination.
Band appearing in the lane of positive control, but not others
Missing band in the lane of positive control
Band appearing in the lane of negative control
Sample was contaminated and the contaminating DNA was amplified.
[Choose]
Template DNA extracted from food sample was degraded or contaminated
with Chelex resin.
[Choose ]
Transcribed Image Text:Identify and match the possible cause(s) of the following unexpected results in agarose gel electrophoresis. (Right minus wrong) Wrong polarity; melted gel; over-run electrophoresis; complete or partial degradation of DNA template. Defective primers. ✓ [Choose ] Entirely empty gel PCR failed, primarily due to missing/degraded template and/or Chelex resi contamination. Band appearing in the lane of positive control, but not others Missing band in the lane of positive control Band appearing in the lane of negative control Sample was contaminated and the contaminating DNA was amplified. [Choose] Template DNA extracted from food sample was degraded or contaminated with Chelex resin. [Choose ]
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