i) Put the steps of a column chromatography analysis in a correct order. a. Elute the column with progressively more polar solvents b. Carefully add the sample mixture to the column as a small volume of solution or liquid, or as a solid adsorbed on silica gel c. Evaporate the solvents to recover the separated compounds d. Prepare a properly packed column of adsorbent e. Collect the eluted compounds in fractions from the column

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i) Put the steps of a column chromatography analysis in a correct order.
a. Elute the column with progressively more polar solvents
b. Carefully add the sample mixture to the column as a small volume of solution
or liquid, or as a solid adsorbed on silica gel
c. Evaporate the solvents to recover the separated compounds
d. Prepare a properly packed column of adsorbent
e. Collect the eluted compounds in fractions from the column
ii) What is the main difference between the gravity column chromatography and the
flash chromatography?
Transcribed Image Text:i) Put the steps of a column chromatography analysis in a correct order. a. Elute the column with progressively more polar solvents b. Carefully add the sample mixture to the column as a small volume of solution or liquid, or as a solid adsorbed on silica gel c. Evaporate the solvents to recover the separated compounds d. Prepare a properly packed column of adsorbent e. Collect the eluted compounds in fractions from the column ii) What is the main difference between the gravity column chromatography and the flash chromatography?
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