I need to make 1 liter of the solution.  I need a list of what I need to use and how much of each. It's the AH solution

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I need to make 1 liter of the solution.  I need a list of what I need to use and how much of each. It's the AH solution 

## Protocol: Visualizing the Beating Heart in Drosophila

### Before You Start

#### Adult Hearts

1. **Preparation of Artificial Hemolymph (AH) Solution**
   - Freshly prepare an artificial hemolymph (AH) solution with the following components:
     - 108mM Na⁺ (Sodium)
     - 5mM K⁺ (Potassium)
     - 2mM Ca²⁺ (Calcium)
     - 8mM MgCl₂ (Magnesium Chloride)
     - 1mM NaH₂PO₄ (Sodium Dihydrogen Phosphate)
     - 4mM NaHCO₃ (Sodium Bicarbonate)
     - 10mM sucrose
     - 5mM trehalose
     - 5mM HEPES (pH 7.1). Ensure all reagents are sourced from Sigma Chemicals.
     - **Note:** The sucrose and trehalose should be added to the AH just before use from refrigerated stock solutions to avoid bacterial contamination.
   
2. **Oxygenation of Artificial Hemolymph**
   - Bring the AH solution to room temperature.
   - Oxygenate the solution by air-bubbling for at least 15 minutes.

### Video and Resources

- The image shows a screenshot from a video demonstrating part of the protocol. You can download the video file [here (122M, mp4)](same_link_description).
- The screenshot displays a beaker filled with a liquid, likely the artificial hemolymph, and this is being prepared following the described protocol steps.

### Additional Information

This protocol is part of a comprehensive guide on visualizing the beating heart in *Drosophila* (fruit flies). For detailed steps and in-depth understanding, refer to the article in the Journal of Visualized Experiments (J Vis Exp. 2009; (31): 1425).

*Ensuring proper preparation and oxygenation of the artificial hemolymph is crucial for the accurate visualization and study of the *Drosophila* heart*.
Transcribed Image Text:## Protocol: Visualizing the Beating Heart in Drosophila ### Before You Start #### Adult Hearts 1. **Preparation of Artificial Hemolymph (AH) Solution** - Freshly prepare an artificial hemolymph (AH) solution with the following components: - 108mM Na⁺ (Sodium) - 5mM K⁺ (Potassium) - 2mM Ca²⁺ (Calcium) - 8mM MgCl₂ (Magnesium Chloride) - 1mM NaH₂PO₄ (Sodium Dihydrogen Phosphate) - 4mM NaHCO₃ (Sodium Bicarbonate) - 10mM sucrose - 5mM trehalose - 5mM HEPES (pH 7.1). Ensure all reagents are sourced from Sigma Chemicals. - **Note:** The sucrose and trehalose should be added to the AH just before use from refrigerated stock solutions to avoid bacterial contamination. 2. **Oxygenation of Artificial Hemolymph** - Bring the AH solution to room temperature. - Oxygenate the solution by air-bubbling for at least 15 minutes. ### Video and Resources - The image shows a screenshot from a video demonstrating part of the protocol. You can download the video file [here (122M, mp4)](same_link_description). - The screenshot displays a beaker filled with a liquid, likely the artificial hemolymph, and this is being prepared following the described protocol steps. ### Additional Information This protocol is part of a comprehensive guide on visualizing the beating heart in *Drosophila* (fruit flies). For detailed steps and in-depth understanding, refer to the article in the Journal of Visualized Experiments (J Vis Exp. 2009; (31): 1425). *Ensuring proper preparation and oxygenation of the artificial hemolymph is crucial for the accurate visualization and study of the *Drosophila* heart*.
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