Ionic Equilibrium
Chemical equilibrium and ionic equilibrium are two major concepts in chemistry. Ionic equilibrium deals with the equilibrium involved in an ionization process while chemical equilibrium deals with the equilibrium during a chemical change. Ionic equilibrium is established between the ions and unionized species in a system. Understanding the concept of ionic equilibrium is very important to answer the questions related to certain chemical reactions in chemistry.
Arrhenius Acid
Arrhenius acid act as a good electrolyte as it dissociates to its respective ions in the aqueous solutions. Keeping it similar to the general acid properties, Arrhenius acid also neutralizes bases and turns litmus paper into red.
Bronsted Lowry Base In Inorganic Chemistry
Bronsted-Lowry base in inorganic chemistry is any chemical substance that can accept a proton from the other chemical substance it is reacting with.
I need to make 1 liter of the solution. I need a list of what I need to use and how much of each. It's the AH solution
![## Protocol: Visualizing the Beating Heart in Drosophila
### Before You Start
#### Adult Hearts
1. **Preparation of Artificial Hemolymph (AH) Solution**
- Freshly prepare an artificial hemolymph (AH) solution with the following components:
- 108mM Na⁺ (Sodium)
- 5mM K⁺ (Potassium)
- 2mM Ca²⁺ (Calcium)
- 8mM MgCl₂ (Magnesium Chloride)
- 1mM NaH₂PO₄ (Sodium Dihydrogen Phosphate)
- 4mM NaHCO₃ (Sodium Bicarbonate)
- 10mM sucrose
- 5mM trehalose
- 5mM HEPES (pH 7.1). Ensure all reagents are sourced from Sigma Chemicals.
- **Note:** The sucrose and trehalose should be added to the AH just before use from refrigerated stock solutions to avoid bacterial contamination.
2. **Oxygenation of Artificial Hemolymph**
- Bring the AH solution to room temperature.
- Oxygenate the solution by air-bubbling for at least 15 minutes.
### Video and Resources
- The image shows a screenshot from a video demonstrating part of the protocol. You can download the video file [here (122M, mp4)](same_link_description).
- The screenshot displays a beaker filled with a liquid, likely the artificial hemolymph, and this is being prepared following the described protocol steps.
### Additional Information
This protocol is part of a comprehensive guide on visualizing the beating heart in *Drosophila* (fruit flies). For detailed steps and in-depth understanding, refer to the article in the Journal of Visualized Experiments (J Vis Exp. 2009; (31): 1425).
*Ensuring proper preparation and oxygenation of the artificial hemolymph is crucial for the accurate visualization and study of the *Drosophila* heart*.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3fda7e25-a6bf-4414-9157-cf31763ef750%2F241fde28-ed1f-4658-9c42-46f4ca022fed%2F0hymsv8_processed.jpeg&w=3840&q=75)
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