I crush up some liver cells and do a series of centrifuge spins at increasing speeds that produce a g-force of 600x, 15,000x, and 100,000x. After each spin, I save the pellet and 5% of the supernatant volume. Then I centrifuge the remaining supernatant. I test each pellet and supernatant for activity in oxidizing Acetyl-CoA to CO2. Which samples will exhibit this activity? lysed cell mixture before 600xg spin supernatant after 600 xg spin supernatant after 15,000 xg spin pellet after 15,000 xg spin (after resuspension of pellet in buffer) supernatant after 100,000 xg spin pellet after 100,000 xg spin (after resuspension of pellet in buffer)
I crush up some liver cells and do a series of centrifuge spins at increasing speeds that produce a g-force of 600x, 15,000x, and 100,000x. After each spin, I save the pellet and 5% of the supernatant volume. Then I centrifuge the remaining supernatant. I test each pellet and supernatant for activity in oxidizing Acetyl-CoA to CO2. Which samples will exhibit this activity? lysed cell mixture before 600xg spin supernatant after 600 xg spin supernatant after 15,000 xg spin pellet after 15,000 xg spin (after resuspension of pellet in buffer) supernatant after 100,000 xg spin pellet after 100,000 xg spin (after resuspension of pellet in buffer)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:I crush up some liver cells and do a series of centrifuge spins at increasing speeds that produce a g-force of 600x, 15,000x, and 100,000x.
After each spin, I save the pellet and 5% of the supernatant volume. Then I centrifuge the remaining supernatant.
I test each pellet and supernatant for activity in oxidizing Acetyl-CoA to CO2.
Which samples will exhibit this activity?
lysed cell mixture before 600xg spin
supernatant after 600 xg spin
supernatant after 15,000 xg spin
pellet after 15,000 xg spin (after resuspension of pellet in buffer)
supernatant after 100,000 xg spin
pellet after 100,000 xg spin (after resuspension of pellet in buffer)
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