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hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX. Earth allowed the chromatograms to develop for around five minutes. Afterwards, he removed the plates from the containers and placed them inside an iodine chamber. The chromatograms resulting from the TLC experiment after visualization are illustrated as follows. The distances travelled by each spot and by the solvents were measured and recorded in the displayed table. 75.0 mm 10.5 mm 0 mm U B A WHITE 71.5 mm solvent front origin. 8 mm 0 mm. B MIX SOLVENT SYSTEM WHITE MIX SPOTS A B C D A B C D DISTANCE TRAVELLED FROM THE ORIGIN, mm 13.0 32.5 41.0 66.5 10.5 27.5 39.0 53.5

hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX. Earth allowed the chromatograms to develop for around five minutes. Afterwards, he removed the plates from the containers and placed them inside an iodine chamber. The chromatograms resulting from the TLC experiment after visualization are illustrated as follows. The distances travelled by each spot and by the solvents were measured and recorded in the displayed table. 75.0 mm 10.5 mm 0 mm U B A WHITE 71.5 mm solvent front origin. 8 mm 0 mm. B MIX SOLVENT SYSTEM WHITE MIX SPOTS A B C D A B C D DISTANCE TRAVELLED FROM THE ORIGIN, mm 13.0 32.5 41.0 66.5 10.5 27.5 39.0 53.5

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Compute for the Rf value 

A. THIN LAYER CHROMATOGRAPHY Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX. Earth allowed the chromatograms to develop for around five minutes. Afterwards, he removed the plates from the containers and placed them inside an iodine chamber. The chromatograms resulting from the TLC experiment after visualization are illustrated as follows. The distances travelled by each spot and by the solvents were measured and recorded in the displayed table. 75.0 mm 10.5 mm 0 mm. C A WHITE 71.5 mm solvent front origin. 8 mm 0 mm A MIX SOLVENT SYSTEM WHITE MIX SPOTS A B C D A B C D DISTANCE TRAVELLED FROM THE ORIGIN, mm 13.0 32.5 41.0 66.5 10.5 27.5 39.0 53.5
Transcribed Image Text:A. THIN LAYER CHROMATOGRAPHY Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX. Earth allowed the chromatograms to develop for around five minutes. Afterwards, he removed the plates from the containers and placed them inside an iodine chamber. The chromatograms resulting from the TLC experiment after visualization are illustrated as follows. The distances travelled by each spot and by the solvents were measured and recorded in the displayed table. 75.0 mm 10.5 mm 0 mm. C A WHITE 71.5 mm solvent front origin. 8 mm 0 mm A MIX SOLVENT SYSTEM WHITE MIX SPOTS A B C D A B C D DISTANCE TRAVELLED FROM THE ORIGIN, mm 13.0 32.5 41.0 66.5 10.5 27.5 39.0 53.5
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