How would you make 0.50L of 250mM buffer at pH 4.30 using only materials you have available in lab?

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
icon
Related questions
icon
Concept explainers
Question
100%
How would you make 0.50L of 250mM buffer at pH 4.30 using only materials you have available in lab?
Show all of your work, including units!
Materials Available in the Lab
DI Water
Acetate pK, = 4.76
1.5 M acetic acid solution
CH3 COOH
Solid sodium acetate (molar mass 82 g/mol)
NACH;C00
Tris (pKa = 8.10)
(HOCH,)3NH;Cl
(HOCH,)3N H2
Solid tris chloride (molar mass 157.6 g/mol)
Solid tris base (molar mass 121.1 g/mol)
Phosphate (pK1 = 2.14, pK2 = 6.86, pK3 = 12.4)
%3D
%3D
%3D
1.0 M phosphoric acid solution
H3PO4
1.5 M monosodium phosphate solution
NaH, PO4
1.25 M disodium phosphate solution
Na, HPO,
2.25 M trisodium phosphate solution
Naz PO4
Transcribed Image Text:Show all of your work, including units! Materials Available in the Lab DI Water Acetate pK, = 4.76 1.5 M acetic acid solution CH3 COOH Solid sodium acetate (molar mass 82 g/mol) NACH;C00 Tris (pKa = 8.10) (HOCH,)3NH;Cl (HOCH,)3N H2 Solid tris chloride (molar mass 157.6 g/mol) Solid tris base (molar mass 121.1 g/mol) Phosphate (pK1 = 2.14, pK2 = 6.86, pK3 = 12.4) %3D %3D %3D 1.0 M phosphoric acid solution H3PO4 1.5 M monosodium phosphate solution NaH, PO4 1.25 M disodium phosphate solution Na, HPO, 2.25 M trisodium phosphate solution Naz PO4
Expert Solution
Step 1

Since pH 4.30 is close to the pka of acetate, so we will make acetate buffer of pH 4.30.

Using Hasselbach-Henderson equation, we will calculate the calculate the concentration of acetic acid and sodium acetate.

steps

Step by step

Solved in 4 steps

Blurred answer
Knowledge Booster
Ionic Equilibrium
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY