Answered: How to prepare wet and dry mount…

How to prepare wet and dry mount specimen for examination. 2. How to care the microscope and step by step procedure on how to use the microscope

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter38: Introduction To The Medical Laboratory

Section: Chapter Questions

Problem 6CR

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1.How to prepare wet and dry mount specimen for examination.

2. How to care the microscope and step by step procedure on how to use the microscope.

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PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observation
3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.
The specimen you are examining is very thin and transparent. What can you do to make it easier to see
Which of the follow are correct statements about microscope handling and care? 1. Always use TWO HANDS to carry the microscope.  2. Only use LENS PAPER to clean the lenses. Never wipe back and forth, but simply make a gentle swipe across the lens. 3. A soft cloth should be used to clean the lenses. 4. Start and finish the day’s work at the LOWEST MAGNIFICATION. 5. Start and finish the day’s work at the HIGHEST MAGNIFICATION 6. Always BEGIN examining a specimen with a LOW POWER objective. 7. Always BEGIN examining a specimen with a HIGHEST POWER objective 8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective. 9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective. 10. Unplug the microscope by pulling on the plug, not the cord.
A. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Power
1. Match: Read about each microscope part. Match the description to the part on the diagram. Stage: Platform where a slide is placed. Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly magnifies a sample by 10x. Coarse focus knob: Large knob that moves the stage up and down to focus the sample. Fine focus knob: Small knob that moves the stage over a short distance to refine the focus. Objective lens: A second lens that further magnifies the sample. Microscopes usually have several objective lenses with different magnifications. Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
1. A microscope is turned on, under low power, with a slide positioned on the stage ready to focus. The specimen is visible but not in focus at all. - write the correct procedural steps used to find and focus on the specimen under medium power.
5) Label the parts of the microscope. 4 1 5 7 8 Nikon 10 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. CO
3 6 9 12 V Stage control knob 1. Mechanical support - is used for carrying the microscope Fine adjustment knob 2. Platform upon which specimen is placed Objective 3. Brings object into approximate focus Diaphragm/Iris 4. Eyepiece/ocular 5. Collects light from source and focuses it upon specimen Controls amount of illumination used to view the object; impacts contrast Stage nation Base 6. Lenses you look into 7. Provides more precise focusing on the object 8. Revolving device that holds the Condenser objective lenses 9. Provides additional magnification of Arm varying powers 10. Mechanical support rests on flat Nosepiece Coarse adjustment knob Stage clip surface of table 11. Hold slides in place 12. 122 Provides mechanical means to move the stage
USING & FOCUSING THE MICROSCOPE: 1. List the following items into their correct order for proper set-up & use of the microscope: set scanning lens (4x), set low power lens (10x), turn on power, place slide on stage, set light to medium, look through eyepiece (= oculars), fine adjustment focus, coarse adjustment focus, center specimen 1. 2. 3. 4. 5. 6. 7. 8. 9.
4. Identify the particular parts on the microscope and indicate what it would be used for. A A B В