How is the gene of interest inserted into the bacteriophage lambda?

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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How is the gene of interest inserted into the bacteriophage lambda?

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Introduction

A bacteriophage, also referred to as a phage colloquially, is a duplodnaviria virus that attacks and replicates inside of bacteria and archaea.

Bacteriophages are made of proteins that enclose a DNA or RNA genome and can have either straightforward or complex structural designs. Their genomes could contain as little as four genes (MS2, for example) or as many as hundreds. The insertion of the phage genome into the cytoplasm of the bacterium initiates phage replication within the bacterium.

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