Answered: How is sperm DNA fragmentation tested?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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How is sperm DNA fragmentation tested?

Expert Solution

Step 1 Introduction:

The integrity of genetic material in the sperm is crucial for successful fertilization and normal embryo development.

Step 2 Concept:

Sperm DNA fragmentation is a term used to denote abnormal genetic material within the sperm, which in turn may lead to male subfertility, IVF failure, and miscarriage. When sperm counts are limited, DNA fragmentation can be effectively assessed using the Comet assay as only 5000 sperm is required. The Comet assay uses electrophoresis to determine abnormal sperm and can measure both single and double-strand breaks.

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