How does a host cell harboring a restriction enzyme protect its own DNA?

Q: How do plasmids cause antibiotic resistance?

A: The plasmids are used to deliver DNA contain genes for antibiotic resistance.

Q: Explain the Retroviral vectors of Gene Therapy ?

A: Retrovirus is used to infect the mammalian cells as they used host cells to multiply. Retrovirus is…

Q: How is mismatch repair different from excision repair (in terms of what type of mutation it is used…

A: In order to prevent and fix damage from mutation, all the cells have a variety of mechanisms to…

Q: How does DNA cloning in a plasmid vector permits amplification of a DNA fragment.

A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…

Q: What is the process known as reverse transcriptase.?

A: 'Reverse' means opposite. Transcription is the process of RNA synthesis from DNA. Transcriptase is…

Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?

A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…

Q: Describe the role that integrase plays during the insertion of λDNA into the host chromosome.

A: Integrase is a viral enzyme that catalyzes the integration of virally derived DNA into host cell…

Q: Why must genetically altered bacteria be grown in a carefully controlled environment?

A: Introduction: The living organisms whose genetic material has been falsely controlled in a lab…

Q: What is the job of the restriction enzyme?

A: In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli…

Q: How is the copy number of plasmid vector and yield of the recombinant protein related to each other?

A: In biotechnology, plasmid refers to the double stranded, circular DNA of bacteria; when these…

Q: What are the functions of relaxase, coupling factor, and the exporter in the process of conjugation?

A: Transferring of genetic material from one bacteria to another by direct contact Relaxase enzyme…

Q: What role does plasmids play in genetic engineering?

A: A plasmid is a small, circular, double-stranded extra chromosomal DNA molecule present within the…

Q: What does the F plasmid code for?

A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…

Q: How is a gene inserted into a plasmid cloning vector?

A: Apart from cell's chromosomal DNA, a double-stranded circular DNA molecule is present in yeast,…

Q: A plasmid provides its host with kanamycin resistance. What will happen to the plasmid if you…

A: Plasmids are defined as extrachromosomal circular genetic elements that are capable of…

Q: What is plasmid DNA used for?

A: Circular DNA is a kind of DNA that makes a closed loop. This kind of DNA has no DNA. They are…

Q: How does specialized transduction differ from generalized transduction?

A: Transduction is a method of gene transfer in bacteria from donor to recipient using bacteriophage.…

Q: Why reverse transcriptase is used ?

A: Viruses can have either DNA or RNA as its genetic material. The DNA or RNA can further be single…

Q: What are the differences between transformation, transfection and transduction?

A: Bacteria are small microscopic prokaryotes , generally ubiquitous . Genetic transfer mechanism…

Q: How Host Cells Take Up and AmplifyRecombinant DNA?

A: Recombinant DNA technology is the joining together of DNA molecules from two different species. The…

Q: Why most genes conferring antibiotic resistance reside on R plasmids in pathogens associated with…

A: Antibiotic resistance is the surviving strategy used by the bacteria to escape antibiotics that are…

Q: How does transduction differ from transformation?

A: Bacteria reproduce to confirm the survival of their species. Every cycle (generation) produces…

Q: What is the role of a vector in gene therapy?

A: Gene therapy is based on the premise that replacement of a disease-causing gene with the healthy…

Q: What is artificial transformation used for?

A: Transformation is a process in which an organism acquires exogenous DNA. This process occurs in two…

Q: What is the importance of methylation in the activity of restriction endonucleases?

A: A restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific…

Q: What is the function of a restriction enzyme?

A: Restriction enzymes are enzymes that are isolated from bacteria that can cleave single-stranded or…

Q: What is the importance of the antibiotic resistance marker gene in the plasmid.

A: ANSWER;- - Adding an anti-microbial obstruction gene to the plasmid tackles the two issues on the…

Q: How do you know if you’re purified plasmids contain your mutation? Why or why not?

A: Purified plasmids often contain mutations that were not intended by the researcher. To determine if…

Q: How does a host cell harboring a restriction enzyme protect its ow DNA?

A: A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or…

Q: What is Base excision repair (BER) ?

A: Any of numerous ways whereby a cell preserves the fidelity of its genetic sequence is known as DNA…

Q: Why are viruses used in gene therapy?

A: Gene therapy is a procedure that modifies a person's genes in order to treat or cure a disease. Gene…

Q: Whats the role of a restriction enzyme?

A: Restriction enzyme is an enzyme used in recombinant DNA technology for the purpose of cloning DNA.…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.