How could you isolate mitochondria from liver cells using differential centrifugation?
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How could you isolate mitochondria from liver cells using differential centrifugation?
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- Three actively growing cultures of the same strain of yeast (saccharomyces cerevisiae) are used. Culture A has been incubated for 48 hours and Culture B has been incubated for 24hours and Culture C for 6 hours. a) Make a drawing of the cells from each culture to identify the various stages of the cell division. b) Is there any difference between the 3 cultures?What cell phases do the alkylating agents work on?a)S phaseb) G0c) G1d) G2e) M phase210 220 230 240 250 260 270 280 290 300 310 320 41 42 43 44 45 460 470 48 49 50 51 As discussed in lab and lecture, Endospores serve an im cells. A) What is the purpose of an endospore? B) Explain the endospores relationship to reproduction. C) What procedure would you use to test for them in lab? For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS Paragraph A & B ¶ BAX 安田图 † {} I ¶ T< 20°F V Arial XQ5d - ABC V 10 == ΠΩ Click Save and Submit to save and submit. Click Save All Answers to sa
- In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result: 1) prototype colonies grew well on minimal medium 2) F+ cells were converted to F- cells despite the physical separation 3)F- cells were converted F+ cells despite the physical separation 4)F+ cells were not converted to F- cells because of the physical separation 5) F- cells were not converted to F+ cells because of the physical separation 6)there was no growth of prototypes on minimal mediumIn the cell wall staining experiment of Bacillus megaterium, what color can you find in a) cell wall and why? b) cytoplasm and why?Which organelle is isolated at the slowest speed on a centrifuge? Which organelle is isolated last after stepwise increase in centrifuge speed?
- 43) The major setback for the use of Transmission Electron Microscope is that: a) You can’t stain the sample with colored dyes b) You can’t see internal structures of the cell c) You can’t focus on Organelles inside the cell d) you can’t focus on cell membranes44) The major advantage of using the Scanning Electron Microscope (SEM) over the Transmission Electron Microscope (TEM) is that: a) You can use live material in looking through the SEM and not the TEM b) You can look at internal structures with SEM & not TEM c) You can use higher magnifications with SEM d) You can look at solid objects with SEM45) The image that you are observing in the SEM comes from: a) Direct reflection/bouncing of electrons from the specimen you are looking at b) Backscattered electros from the secondary electrons from the specimen c) A contrast between the texture of the specimen you are looking at d) A contrast between the acidophilic and basophilic components of the specimen you are looking at46) A…What cell types would be able to grow on the ECM in the following situations?a) Streptomycin was not added to the ECM.b) The ECM contains thiamine.c) The ECM contains all 20 amino acids and all 5 nitrogenous nucleic acid bases. Reminder: ECM = minimal medium + glucose + has streptomycin antibioticYou wish to identify the presence of a specific protein inside the cell. What microscopy technique should you use? a) Transmission electron microscopy b) Light microscopy Oc) Scanning electron microscopy d) Fluorescent microscopy e) None of the above
- If the generation time of Escherichia coli is 30 minutes, starting with 4 E. coli cells, how many cells can you obtain after 2 hours of growth? A: cellsFrom which microbe did mitochondria arise? What is the reason behind this belief?Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences?
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