How could you have made 10 μL of the 50 ng/μL of DNA solution directly from the 500 ng/μLstock?

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question #9

How could you have made 10 μL of the 50 ng/μL of DNA solution directly from the 500 ng/μLstock?

Expert Solution
Step 1

The given problem relates to stock dilution, so we can use the below relation to calculate the volume of stock solution taken to dilute it to another required concentration. It is given by,                                                   

                                                  S1V1 = S2V

    Where, S1 is the initial concentration

                V1 is the initial volume of stock solution taken.

                S2 is the final concentration

                V2 is the final volume of the solution

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