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How can I found the log of molecular weight ? Please

How can I found the log of molecular weight ? Please

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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How can I found the log of molecular weight ? Please
coming the Gel (during second week of lab) 1. Pour off Coomassie stain into the designated container. 2. Wash gel carefully with water to remove excess stain. 3. Add Destain solution. Gently rock gel for one or two hours. F. Analyzing the Gel 1. Pour destain into waste container. Add approximately 200mL of water to gel box. Gel may curl up for a few minutes but will eventually become flat again. 2. Place plastic wrap on the surface of the light box. Carefully transfer your gel to the plastic wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel with a layer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = 54 mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the KD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Rf= Distance Band Migration (mm) Dye Front Migration 0-074 d 17 22. 28. 31 34 0-111 0-166 0.203 0.314 0-407 esig ܡܘܢ ܥܘ 0.759, Measurements for Unknown A Migration Distance (mm) Molecular Weight (in KD) 260 250. Rf 8123see methods Steps Results DISCLusion Analysis. Conclusion or Reface. 75 50 kDa -~-260 --140- --100- - -70 -50 -40 -35 -25 -15 ~10 Log of Molecular Weight 2.415 Estimated Molecular Weight
Transcribed Image Text:coming the Gel (during second week of lab) 1. Pour off Coomassie stain into the designated container. 2. Wash gel carefully with water to remove excess stain. 3. Add Destain solution. Gently rock gel for one or two hours. F. Analyzing the Gel 1. Pour destain into waste container. Add approximately 200mL of water to gel box. Gel may curl up for a few minutes but will eventually become flat again. 2. Place plastic wrap on the surface of the light box. Carefully transfer your gel to the plastic wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel with a layer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = 54 mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the KD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Rf= Distance Band Migration (mm) Dye Front Migration 0-074 d 17 22. 28. 31 34 0-111 0-166 0.203 0.314 0-407 esig ܡܘܢ ܥܘ 0.759, Measurements for Unknown A Migration Distance (mm) Molecular Weight (in KD) 260 250. Rf 8123see methods Steps Results DISCLusion Analysis. Conclusion or Reface. 75 50 kDa -~-260 --140- --100- - -70 -50 -40 -35 -25 -15 ~10 Log of Molecular Weight 2.415 Estimated Molecular Weight
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