Answered: how can denaturing environment be…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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how can denaturing environment be created during electrophoresis? which bonds are broken during DNA denaturation?

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Definitions:

Electrophoresis:

Electrophoresis is defined as a laboratory technique that involves the separation of DNA, RNA and Protein molecules depending on size and charge.

In this separation process, the molecules are forced to travel across an electric field through a gel with small pores.

Denaturing gel-electrophoresis:

Denaturing will denature the DNA/RNA or proteins to a string of nucleic acids or amino acids respectively.

It is the most sensitive mutation detection method with 99% efficiency.

How a denaturing environment is created?

Denaturing environment is created by applying a uniform run temperature of 50 to 60c and, Urea and formamide are used to form a linear denature gradient.

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