Histology- Upon reviewing routinely stained slides submitted for examination, it was observed that some cases showed evidence of:- patchy staining of slides, inadequate counterstaining, cloudy appearance of slides, and sections appearing partially cut. Analyze these observations, indicate possible sources/causes of the “errors”, and discuss the Quality Control measures that should be implemented to ensure that accurate slides of good quality are produced.
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Biology
Histology- Upon reviewing routinely stained slides submitted for examination, it was observed that some cases showed evidence of:- patchy staining of slides, inadequate counterstaining, cloudy appearance of slides, and sections appearing partially cut. Analyze these observations, indicate possible sources/causes of the “errors”, and discuss the Quality Control measures that should be implemented to ensure that accurate slides of good quality are produced.
Step by step
Solved in 3 steps