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A. Experimental Results Sample No. 1 2 3 4 5 *Enter qualitative observations Table 1. Results of the Qualitative Tests of Carbohydrate Samples Barfoed's Test Aqua blue solution Brick-red precipitates Aqua blue solution Brick-red precipitates Aqua blue solution Seliwanoff's Test Colorless solution Cherry red solution Colorless solution Colorless solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue solution Rust-colored precipitates Colorless solution Aqua blue solution Table 2. Matching of Samples with Reaction Profile Rationale Sample No. Sample Identity 1 2 3 4 5 Lugol's Test Brown solution Brown solution Blue-black solution Brown solution Brown solution

A. Experimental Results Sample No. 1 2 3 4 5 *Enter qualitative observations Table 1. Results of the Qualitative Tests of Carbohydrate Samples Barfoed's Test Aqua blue solution Brick-red precipitates Aqua blue solution Brick-red precipitates Aqua blue solution Seliwanoff's Test Colorless solution Cherry red solution Colorless solution Colorless solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue solution Rust-colored precipitates Colorless solution Aqua blue solution Table 2. Matching of Samples with Reaction Profile Rationale Sample No. Sample Identity 1 2 3 4 5 Lugol's Test Brown solution Brown solution Blue-black solution Brown solution Brown solution

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Qualitative Reactions of Carbohydrates: Matching of Samples with Reaction Profile

1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.
Transcribed Image Text:1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.
A. Experimental Results Sample No. 1 2 3 4 5 * Enter qualitative observations Sample No. 1 2 3 نيا A 5 Table 1. Results of the Qualitative Tests of Carbohydrate Samples Barfoed's Test Aqua blue solution Brick-red precipitates Aqua blue solution Brick-red precipitates Aqua blue solution Seliwanoff's Test Colorless solution Cherry red solution Colorless solution Colorless solution Colorless solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue solution Rust-colored precipitates Aqua blue solution Table 2. Matching of Samples with Reaction Profile Sample Identity Rationale Lugol's Test Brown solution Brown solution Blue-black solution Brown solution Brown solution
Transcribed Image Text:A. Experimental Results Sample No. 1 2 3 4 5 * Enter qualitative observations Sample No. 1 2 3 نيا A 5 Table 1. Results of the Qualitative Tests of Carbohydrate Samples Barfoed's Test Aqua blue solution Brick-red precipitates Aqua blue solution Brick-red precipitates Aqua blue solution Seliwanoff's Test Colorless solution Cherry red solution Colorless solution Colorless solution Colorless solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue solution Rust-colored precipitates Aqua blue solution Table 2. Matching of Samples with Reaction Profile Sample Identity Rationale Lugol's Test Brown solution Brown solution Blue-black solution Brown solution Brown solution
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