H :0: OFF H N-H : OH NANON N-CH3 :0: HO: GUN :0: :0: OHM :0: :N-CH H₂C TAY

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Kate wanted to use TLC to separate the constituents of a leaf extract from the plant
Plumeria pudica. Louie initially prepared his adsorbent by allowing a silica gel slurry to
solidify on a glass slide. After the plate has been prepared, Louie uses a mortar and pestle
and dichloromethane as an extracting solvent to crush the plant's leaves. Then, he spotted
his prepared extract on the hydrophobic silica TLC plate, which he then placed in an
equilibrated developing chamber containing 6:2:2 (v/v/v) water: acetone: ethyl acetate.
The chromatogram was then allowed to develop for 5 minutes. Finally, he drew an
illustration of his chromatogram, which is depicted below. Match each spot with its most
possible identity. Calculate the rf values also.
Transcribed Image Text:Kate wanted to use TLC to separate the constituents of a leaf extract from the plant Plumeria pudica. Louie initially prepared his adsorbent by allowing a silica gel slurry to solidify on a glass slide. After the plate has been prepared, Louie uses a mortar and pestle and dichloromethane as an extracting solvent to crush the plant's leaves. Then, he spotted his prepared extract on the hydrophobic silica TLC plate, which he then placed in an equilibrated developing chamber containing 6:2:2 (v/v/v) water: acetone: ethyl acetate. The chromatogram was then allowed to develop for 5 minutes. Finally, he drew an illustration of his chromatogram, which is depicted below. Match each spot with its most possible identity. Calculate the rf values also.
E
D
C
B
A
H
:0:
OFF
H
N-H
: OH
NANON
N-CH3
:0:
HO:
GUN
:0:
:0:
OHM
:0:
:N-CH3
H3C
TAY
Transcribed Image Text:E D C B A H :0: OFF H N-H : OH NANON N-CH3 :0: HO: GUN :0: :0: OHM :0: :N-CH3 H3C TAY
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