Glucolate is oxidized into Glvoxylate by cytechrome C using the glycolate oxidase enzyme. The two half- reactions are shown below in their standard reduction forms. но +2 H* (aq) + 2 e Glyoxylate Glycolate
Glucolate is oxidized into Glvoxylate by cytechrome C using the glycolate oxidase enzyme. The two half- reactions are shown below in their standard reduction forms. но +2 H* (aq) + 2 e Glyoxylate Glycolate
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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4
![2.
Glvcolate is oxidized into Glvoxylate by cytochrome C using the glycolate oxidase enzyme. The two half-
reactions are shown below in their standard reduction forms.
но
+2 H* (aq) + 2 e
Glyoxylate
Glycolate
CytC-Fe* +e
CytC-Fe
a) What is the overall reaction in the enzyme redox reaction?
b) Given that the (i) equilibrium constant at pH-7 and 25 C for the overall reaction in (a) is 2.14x10", and (i) the
biochemical standard reduction potential of cytochrome C is +0.25 V, calculate the overall biochemical standard
voltage at the enzyme (E") and the biochemical standard reduction potential of glyoxylate.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F02728e8c-0e0f-4e37-a55e-e2f67feb1ff9%2F62f5b7ff-837a-41f1-876a-a037c75044d6%2Fm1l2wjl_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2.
Glvcolate is oxidized into Glvoxylate by cytochrome C using the glycolate oxidase enzyme. The two half-
reactions are shown below in their standard reduction forms.
но
+2 H* (aq) + 2 e
Glyoxylate
Glycolate
CytC-Fe* +e
CytC-Fe
a) What is the overall reaction in the enzyme redox reaction?
b) Given that the (i) equilibrium constant at pH-7 and 25 C for the overall reaction in (a) is 2.14x10", and (i) the
biochemical standard reduction potential of cytochrome C is +0.25 V, calculate the overall biochemical standard
voltage at the enzyme (E") and the biochemical standard reduction potential of glyoxylate.
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