Given the data for Chymosin shown below: What are the catalytic constants (i.e. Km and Vmax) for these two reactions (+I and −I)?; and Draw the Lineweaver-Burk plots and determine the type of inhibition that is indicated. Sample [S] # 1 2 3 4 5 (μM) 6.0 10.0 20.0 60.0 180.0 V (μmol/min.) no I 20.80 29.00 45.00 67.60 87.00 V (μM/min.) with Inhib. 4.20 5.80 9.00 13.60 16.20
Given the data for Chymosin shown below: What are the catalytic constants (i.e. Km and Vmax) for these two reactions (+I and −I)?; and Draw the Lineweaver-Burk plots and determine the type of inhibition that is indicated. Sample [S] # 1 2 3 4 5 (μM) 6.0 10.0 20.0 60.0 180.0 V (μmol/min.) no I 20.80 29.00 45.00 67.60 87.00 V (μM/min.) with Inhib. 4.20 5.80 9.00 13.60 16.20
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![I.
2.
Given the data for Chymosin shown below:
What are the catalytic constants (i.e. Km and Vmax) for
these two reactions (+I and −I)?; and
Draw the Lineweaver-Burk plots and determine the type
of inhibition that is indicated.
Sample [S]
#
1
2
3
4
5
(μM)
6.0
10.0
20.0
60.0
180.0
V (μmol/min.) no I
20.80
29.00
45.00
67.60
87.00
V (UM/min.) with Inhib.
4.20
5.80
9.00
13.60
16.20](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4c5addd0-3107-4aed-8c6b-6b5b2f9fadb0%2Ff46537f9-a8e9-4c52-965c-350b5b8d876d%2Fodcw69r_processed.jpeg&w=3840&q=75)
Transcribed Image Text:I.
2.
Given the data for Chymosin shown below:
What are the catalytic constants (i.e. Km and Vmax) for
these two reactions (+I and −I)?; and
Draw the Lineweaver-Burk plots and determine the type
of inhibition that is indicated.
Sample [S]
#
1
2
3
4
5
(μM)
6.0
10.0
20.0
60.0
180.0
V (μmol/min.) no I
20.80
29.00
45.00
67.60
87.00
V (UM/min.) with Inhib.
4.20
5.80
9.00
13.60
16.20
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