Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.300 M HCl, 0.300 M NaOH and distilled water, describe the preparation of 1 L of 0.0500 M anserine buffered solution, pH 7.20. Volume of stock solution needed =___ mL Volume of HCl needed to adjust the imidazole group = ___ mL Volume of HCl needed to titrate the amino group = ___mL Total HCl needed = ___ mL.
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Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.300 M HCl, 0.300 M NaOH and distilled water, describe the preparation of 1 L of 0.0500 M anserine buffered solution, pH 7.20.
Volume of stock solution needed =___ mL
Volume of HCl needed to adjust the imidazole group = ___ mL
Volume of HCl needed to titrate the amino group = ___mL
Total HCl needed = ___ mL.
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