For linkage analysis, a test cross is used rather than a hybrid cross. Why is this essential? Why would a hybrid cross resul in incorrect estimates of genetic distance?
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- Does a molecular marker have to be polymorphic to be useful in physical mapping studies? Does a molecular marker have to be polymorphic to be useful in linkage mapping (i.e., involving family pedigree studies or genetic crosses)? Explain why or why not.You are studying three linked genes in snapdragons. The flower color locus is in the center. There are 12.7 cM between the flower color locus and the plant height locus. There are 8.8 cM between the flower color locus and the leaf type locus. The coefficient of coincidence is 0.8. Pure-breeding tall, red-flowered plants with fuzzy leaves were crossed to pure-breeding dwarf, blue-flowered plants with smooth leaves. The F1 were testcrossed. Calculate the proportion of the testcross progeny that are expected to have blue flowers and smooth leaves. Round properly to 4 decimal digits. AnswerYou are studying three linked genes in snapdragons. The flower color locus is in the center. There are 8.3 cM between the flower color locus and the plant height locus. There are 12.4 cM between the flower color locus and the leaf type locus. The coefficient of coincidence is 0.8. Pure-breeding tall, red-flowered plants with fuzzy leaves were crossed to pure-breeding dwarf, blue-flowered plants with smooth leaves. The F1 were testcrossed. Calculate the proportion of the testcross progeny that are expected to have red flowers and fuzzy leaves. Round properly to 4 decimal digits. Answer:
- Why is it more efficient to perform a test cross with a homozygous recessive donor than a homozygous dominant donor? How could the same information still be found with a homozygous dominant donor?Two linked loci have a recombination frequency of 5%. A series of crosses is performed. The P generation includes an individual that is homozygous dominant for trait 1 and homozygous recessive for trait 2. The second individual is homozygous recessive for trait 1 and homozygous dominant for trait 2. The F1 generation is crossed with individuals that are homozygous recessive for both traits. If 400 F2 offspring are produced, how many offspring with each phenotype are expected? Fill in the table below with your answers. Phenotype Number of Offspring Predicted Recessive 1, Recessive 2 Dominant 1, Dominant 2 Recessive 1, Dominant 2 Dominant 1, Recessive 2 Total offspring 400 For the results above, determine which phenotypes are parental and which are recombinant. Phenotype Parental or Recombinant? Dominant 1, Dominant 2 Recessive 1, Dominant 2 Recessive 1, Recessive 2 Dominant 1, Recessive 2Solve the following problem by listing the given genotypes, test crosses, and genotypic and phenotypic ratios. A plant with serrated leaf margins is crossed with a plant with smooth leaf margins, producing a plant with wavy leaf margins. What will be the phenotypic and genotypic ratios if a homozygous plant with serrated leaves is crossed with a plant with wavy leaves?
- What is the most convenient way of understanding a testcross problem in genetics?You have already localized the genes to the same chromosome by deletion mapping, and now decide that the best way to accomplish the mapping is to conduct two simultaneous three-point testcross experiments. The genes you are investigating are as follows: N = round leaves, n = notched leaves; H = smooth stems, h = hairy stems; R = purple flowers, r = red flowers; B = grey seeds, b = black seeds; and Y = green pods, y = yellow pods. Earlier experiments you have done already established that gene B is in the middle of this gene cluster, so you design both three-point test crosses to include that gene. Cross #1 is designed as RrHhBb x rrhhbb while cross #2 is NnBbYy x nnbbyy. The results of both crosses are given in the table below. Based on the information given, determine the arrangement of these five genes including the position of each allele in the heterozygous fly and the distances between each pair of genes. (Hint: treat each experiment separately, knowing that gene B is in the…To determine the recombination frequency between body color and wing genes in flies, you perform several crosses where you cross an F1 having red body and smooth wings with a yellow-bodied, crinkle-winged fly. You get the following results. What is the distance between the genes for body color and wing surface in map units?
- An ebony strain of flies was discovered to be sensitive to carbon dioxide. Crossing a female sensitive strain with male resistant strain gave all sensitive offspring. The offspring of an F1 female crossed with a resistant male were all sensitive. Using the following key to your illustrations using shapes, make a reciprocal cross up to the F2 generation. Put your illustrations in the space provided below. Label the phenotypes of all individuals in the reciprocal cross. Adjust spacing, if necessary. Make sure that the complete cross(es) can fit the same page. Big blue circle - male cytoplasm Big pink circle - female cytoplasm Small half-blue-half-pink circle - F1 nucleus Small blue circle - male nucleus Small pink circle - female nucleus give a diagram pleaseAn ebony strain of flies was discovered to be sensitive to carbon dioxide. Crossing a female sensitive strain with male resistant strain gave all sensitive offspring. The offspring of an F1 female crossed with a resistant male were all sensitive. Using the following key to your illustrations using shapes, make a reciprocal cross up to the F2 generation. Put your illustrations in the space provided below. Label the phenotypes of all individuals in the reciprocal cross. Adjust spacing, if necessary. Make sure that the complete cross(es) can fit the same page. Big blue circle - male cytoplasm Big pink circle - female cytoplasm Small half-blue-half-pink circle - F1 nucleus Small blue circle - male nucleus Small pink circle - female nucleusIndividuals of genotype AaBb were mated to individuals of genotype aabb. One thousand offspring were counted, with the following results: 474 Aabb, 480 aaBb, 20 AaBb, and 26 aabb. What type of cross is it? Are these loci linked? What are the two parental classes and the two recombinant classes of offspring? What is the percentage of recombination between these two loci? How many map units apart are they?