For each mode of bioreactor operation listed below, name one advantage and one disadvantage. a. Batch b. Fed-batch c. Continuous
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For each mode of bioreactor operation listed below, name one advantage and one disadvantage.
a. Batch
b. Fed-batch
c. Continuous
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- Describe and discuss which factors influence the choice of bioreactor design?(in long explanation please)Explain briefly effects and importance of following phenomena on bioreactor design. oxygen demand foam production shear and viscosity growth rate pHWhat is biocompatibility? Briefly describe how the success of a biomaterial is evaluated.
- What are the disadvantages of closed bioreactor system?Write down the overview of process of manufacturing of IFN-based biopharmaceuticals. Explain with the help of flow chart or diagram.the 10 mg/mL sample of lysozyme shows a lower activity than 2mg/mL lysozyme sample (refer to the graph shown below). It is potentially due to Inefficiency of lysozyme at high concentrations. Highest concentrated lysozymes do have lowest activity. Loss of data due to user inefficiency. Loss of data due to spectrophotometer inefficiency.
- In an experiment, three batches of the same enzyme were incubated with 5 mM of substrate and 2 mM of inhibitor. In each batch, the inhibitor was of a different type: a.Batch 1: competitive inhibitor b.Batch 2:uncompetitive inhibitor c.Batch 3:non-competitive inhibitor Assuming that Vmax = 80 mM s', Km = 8 mM, and Kiof all three inhibitors was 10 mM, which of the inhibitors was the most efficient on Vo? Explain your answer.With the aid of diagrams, explain the processes and principles of each of the following methods: a. Single substrate in vitro kinetic reaction catalyzed by a non-regulatory enzyme (E) in which there is an increase in the concentration of substrate (S) with time. b. Salting out of proteins.Describe the bioreactor design consideration to be taken in account during the bioprocessing of animal cell culture.