FIGURE 16-17 Summary of the procedure The Ames test reveals mutagenic compounds used for the Ames test. Solubilized liver enzymes (S9) are added to a suspension of auxotrophic bacteria in a solution of the potential carcinogen (X). The mixture is plated on a medium containing no histidine. The presence of revertants indicates that the chemical is a mutagen and possibly a carcinogen as well. His- - Hist reversion test s9 Strain S9 S9 Strain 2 FIGURE 16-18 TA100, TA1538, and TA1535 are strains of Salmonella bearing different histidine auxotrophic mutations. The TA100 strain is highly sensitive to reversion through base-pair substitution. The TA1535 and TA1538 strains are Liver Grind liver and centrifuge enzymes Strain 1 or S9 strain 2 (Control) sensitive to reversion through frameshift mutation. The test results show that His-- His+ reversion test aflatoxin B, is a potent mutation that causes base-pair substitutions but not frameshifts. [Data from J. McCann and B. N. Mix and plate Ames, in W. G. Flamm and M. A. Mehlman,
FIGURE 16-17 Summary of the procedure The Ames test reveals mutagenic compounds used for the Ames test. Solubilized liver enzymes (S9) are added to a suspension of auxotrophic bacteria in a solution of the potential carcinogen (X). The mixture is plated on a medium containing no histidine. The presence of revertants indicates that the chemical is a mutagen and possibly a carcinogen as well. His- - Hist reversion test s9 Strain S9 S9 Strain 2 FIGURE 16-18 TA100, TA1538, and TA1535 are strains of Salmonella bearing different histidine auxotrophic mutations. The TA100 strain is highly sensitive to reversion through base-pair substitution. The TA1535 and TA1538 strains are Liver Grind liver and centrifuge enzymes Strain 1 or S9 strain 2 (Control) sensitive to reversion through frameshift mutation. The test results show that His-- His+ reversion test aflatoxin B, is a potent mutation that causes base-pair substitutions but not frameshifts. [Data from J. McCann and B. N. Mix and plate Ames, in W. G. Flamm and M. A. Mehlman,
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
In the Ames test shown in Figure 16-17, what is the reason for adding the liver extract to each sample?
Transcribed Image Text:FIGURE 16-17 Summary of the procedure
The Ames test reveals mutagenic compounds
used for the Ames test. Solubilized liver
enzymes (S9) are added to a suspension of
auxotrophic bacteria in a solution of the
potential carcinogen (X). The mixture is
plated on a medium containing no histidine.
The presence of revertants indicates that
the chemical is a mutagen and possibly a
carcinogen as well.
His- - Hist
reversion test
s9
Strain
S9
S9
Strain
2
FIGURE 16-18 TA100, TA1538, and
TA1535 are strains of Salmonella bearing
different histidine auxotrophic mutations.
The TA100 strain is highly sensitive to
reversion through base-pair substitution.
The TA1535 and TA1538 strains are
Liver
Grind
liver
and
centrifuge
enzymes
Strain 1
or
S9
strain 2
(Control)
sensitive to reversion through frameshift
mutation. The test results show that
His-- His+
reversion test
aflatoxin B, is a potent mutation that
causes base-pair substitutions but not
frameshifts. [Data from J. McCann and B. N.
Mix and plate
Ames, in W. G. Flamm and M. A. Mehlman,
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