f) As ATP is used, the above, at what ratio of equilibrium)? phosphocreatine stores are depleted. Using the ATP and ADP concentrations given [creatine]/[phosphocreatine] would you expect the reaction to stop (or reach G) do some research. How does the level of phosphocreatine remain high in muscle cells. Where/how is it remade from creatine? (one paragraph)
f) As ATP is used, the above, at what ratio of equilibrium)? phosphocreatine stores are depleted. Using the ATP and ADP concentrations given [creatine]/[phosphocreatine] would you expect the reaction to stop (or reach G) do some research. How does the level of phosphocreatine remain high in muscle cells. Where/how is it remade from creatine? (one paragraph)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
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![Energy Conversion Problem - 2022
1. a) Influence of concentration on the free energy change. In frog muscle, the concentrations of ATP, ADP,
and phosphate are 1.25x10-³ M, 0.50x10-³ M, and 2.50 x10-³ M respectively. Write the reaction and the reaction
quotient, Q. Calculate the free energy change, AG', for the hydrolysis of ATP in muscle cells. Assume that
AG"=-31.0 kJ mol-¹ for the hydrolysis of ATP, the temperature is 25°C, and the pH=7.
Q=(0.50x10-³ M)(1.25x10-³ M)/(2.50 x10-³ M) = 1.25 x 10-6 M/1.25 x 10-³ M = 1 x 10-³ M
A Gº'= -31.0 kJ/mol + (8.314 kJ /mol K_) (298 K) ( [0.5 × 10-³] [2.5 × 10-³] / [1.25 × 10-³])= −48.1 kJ/mol
b) For this system, what is the maximum amount of work that can be done per mole of ATP hydrolyzed?
W max, by the system =-W max, on the surroundings = -ArG' = 48.1 kJ/mol
c) In muscle, phosphocreatine serves as a carrier of chemical energy. It can transfer its phosphate group to ADP
to replenish the ATP used in muscle contraction. This is an example of energy coupling- using the energy of
a very favorable reaction to drive an unfavorable reaction. The enzyme creatine phosphokinase catalyzes
the reaction:
Net rxn
creatine phosphokinase
phosphocreatine + ADP
d) Show above how you calculate AGº' for the net reaction given that the hydrolysis for phosphocreatine is
AG0¹ -43.1 kJ mol-¹.
e) Calculate the equilibrium constant of this reaction.
phosphocreatine + H2O → creatine + Pi
Pi + ADP → ATP + H2O
= -43.1 kJ mol
= 31.0 kJ mol
creatine + ATP
bosphocreatine + ADP → creatine + ATP = -12.1 kJ mol
K-e 12.1 kj/mol / (8.314 x 10-³ kj/mol k) (298K)
K-e 4.89
K= 132.4
AG⁰¹ ???? kJ mol-1
f) As ATP is used, the phosphocreatine stores are depleted. Using the ATP and ADP concentrations given
above, at what ratio of [creatine]/[phosphocreatine] would you expect the reaction to stop (or reach
equilibrium)?
G) do some research. How does the level of phosphocreatine remain high in muscle cells. Where/how is it
remade from creatine? (one paragraph)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F119d1bed-9b12-43b4-801e-dd3337ce3687%2Fa8ea649f-9461-455c-92a9-b6508e2547fe%2Fnu8jnak_processed.png&w=3840&q=75)
Transcribed Image Text:Energy Conversion Problem - 2022
1. a) Influence of concentration on the free energy change. In frog muscle, the concentrations of ATP, ADP,
and phosphate are 1.25x10-³ M, 0.50x10-³ M, and 2.50 x10-³ M respectively. Write the reaction and the reaction
quotient, Q. Calculate the free energy change, AG', for the hydrolysis of ATP in muscle cells. Assume that
AG"=-31.0 kJ mol-¹ for the hydrolysis of ATP, the temperature is 25°C, and the pH=7.
Q=(0.50x10-³ M)(1.25x10-³ M)/(2.50 x10-³ M) = 1.25 x 10-6 M/1.25 x 10-³ M = 1 x 10-³ M
A Gº'= -31.0 kJ/mol + (8.314 kJ /mol K_) (298 K) ( [0.5 × 10-³] [2.5 × 10-³] / [1.25 × 10-³])= −48.1 kJ/mol
b) For this system, what is the maximum amount of work that can be done per mole of ATP hydrolyzed?
W max, by the system =-W max, on the surroundings = -ArG' = 48.1 kJ/mol
c) In muscle, phosphocreatine serves as a carrier of chemical energy. It can transfer its phosphate group to ADP
to replenish the ATP used in muscle contraction. This is an example of energy coupling- using the energy of
a very favorable reaction to drive an unfavorable reaction. The enzyme creatine phosphokinase catalyzes
the reaction:
Net rxn
creatine phosphokinase
phosphocreatine + ADP
d) Show above how you calculate AGº' for the net reaction given that the hydrolysis for phosphocreatine is
AG0¹ -43.1 kJ mol-¹.
e) Calculate the equilibrium constant of this reaction.
phosphocreatine + H2O → creatine + Pi
Pi + ADP → ATP + H2O
= -43.1 kJ mol
= 31.0 kJ mol
creatine + ATP
bosphocreatine + ADP → creatine + ATP = -12.1 kJ mol
K-e 12.1 kj/mol / (8.314 x 10-³ kj/mol k) (298K)
K-e 4.89
K= 132.4
AG⁰¹ ???? kJ mol-1
f) As ATP is used, the phosphocreatine stores are depleted. Using the ATP and ADP concentrations given
above, at what ratio of [creatine]/[phosphocreatine] would you expect the reaction to stop (or reach
equilibrium)?
G) do some research. How does the level of phosphocreatine remain high in muscle cells. Where/how is it
remade from creatine? (one paragraph)
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