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Q: What is the advantage to transferring proteins bands from a gel to nitrocellulose during the western…
A: The western blot is a broadly utilized scientific procedure in molecular biology and immunogenetics…
Q: RT-PCR can determine the amount of mRNA for a particular gene in two different growth conditions.
A: Reverse transcriptase polymerase chain reaction (RT-PCR) is a laboratory technique combining…
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Q: Describe how Blue/White screening reagent helps in screening the colonies of transformed cells.
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A:
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pls do not copy paste
Explain the steps of preparing a cell culture stock from a T25 flask.
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- Compare and contrast the principle behind DNA migration in agarose gel electrophoresis from that of protein migration in SDS-PAGE.Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISHwhat are two differences between agarose gel electrophoresis and SDS PAGE?
- About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.Describe how Blue/White screening reagent helps in screening the colonies of transformed cells.
- A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 4 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 21 plaques. What is the initial density of bacteriophages in the original 1 mL? Recall that initial phage density = (plaque number/mL) ×× (dilution factor).Compare the principle behind DNA migration in an agarose gel electrophoresis from thatof protein migration in an SDS-PAGE.DO NOT COPY IN GOOGLE OR BARTLEBY QUESTION: - Are cells grown in the laboratory will function similarly when transplanted?
- Besides agarosc gel electrophoresis, there is also SDS-PAGE for separation of macromolccules. Differentiate both these methods.Give typed full explanation Antibiotic sensitivity.What is relationship to ellipse size and sensitivity for an E-Test?Select all that may apply. What is the purpose of incubating the lambda phage/E.coli mixture at room temperature for 20min? Incubation at room temperature inactivates the cI repressor. During incubation at room temperature the lambda phage will enter the lytic cycle. Incubation at room temperature allows for the absorption of lambda phage Incubation at room temperature will allow the lambda phage to remain in the lysogenic cycle.