Explain the principle involved in O.D determination of microbial populations using the spectrophotometer

Q: ORGANISMS: STENOTROPHOMONAS MALTOPHILIA ​​Outline the presumptive and definitive…

A: Stenotrophomonas maltophilia is a Gram-negative, aerobic bacterium. Initially it is called as  as…

Q: What are the expected problems in the centrifuge in case of imbalance of samples ? *

A: Centrifugation is a process of separation of molecules from mixtures by applying centrifugal force…

Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?

A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…

Q: please write an introduction on the effects of ph on Microbial Growth

A: pH is defined as a negative logarithm of hydrogen ion concentration.For the proper growth of…

Q: Discuss، Factors that influencing the cleaning process ?

A: There are five main factors which are equally critical when cleaning:time, temperature, mechanical…

Q: With the aid of a labelled diagram, outline the principle of the oil immersion objective

A: A microscope is an intrument which helps to enlarge microscopic images which are not visible to…

Q: Another method of microbial control is filtration. List down at least 5 kinds of filters which could…

A: Filterationn sterilization It is the removal of microbes by the passage of a liquid or gas through a…

Q: define both selective and enrichment media as they may be used in clinical microbiology

A: Media in microbiology or also known as bacterial culture media. It is a growth medium used to grow…

Q: Using pasteurization techniques as a model, compare the TDTs and explain the relationships between…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Explain the imporance of using a control tube with only broth when measuring turbidity in a…

A: Introduction :- There are two types of growth medium or media which are used to grow microorganisms…

Q: explain why it is important to maintain sterility when inserting a foley cathether

A: Foley's catheter is an indwelling catheter, i.e. it can be left in place for longer durations than…

Q: Briefly explain how the API (Analytical Profile Index) system works.

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: provide at least one example where each of these micrscope ( SEM and TEM)  would be the method of…

A: The power of electron microscopes has grown as well. They are an exceedingly effective instrument…

Q: Why is the membrane filter method preferred to the most probable numbermethod (MPN) for the…

A: Most probable number (MPN) is a method used to estimate the numbers of bacterial cells in a water…

Q: Explain the reason for the use of different medium blanks in adjusting thespectrophotometer prior to…

A: Different medium for the growth of bacteria have different components. Due to presence of different…

Q: By the means of gas stoichiometry, how soaps are created?

A: Soap is made up of animal fat or plant oil, as well as caustic soda. It dislodges dirt from surfaces…

Q: Which specific example of a functional medium would you use to grow microbes from the following…

A: A functional medium refers to a cultured medium that is specifically designed to support the growth,…

Q: Describe the shape and color of the following species of bacteria after Acid-Fast staining: E. coli…

A: Acid fast staining is a type of laboratory test that is used to determine if a sample of blood or…

Q: Explain why you can use OD675 (optical density at a wavelength of 675nm) as a proxy for cell density…

A: Algae are group of predominately aquatic, photosynthetic, and nucleus bearing organism lacking true…

Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…

A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…

Q: Outline the most signifi cant discoveries and events in microscopy, culture techniques, and other…

A: Microbes are minute, unicellular/multicellular, prokaryotic living organisms that cannot be seen…

Q: Why is reproducibility of pipetting critical in performing serial dilutions?

A: Introduction :- The movement of liquids from one place to another would be risky and messy without a…

Q: Write down a detailed decription of the most important configurations in which the mass analisers in…

A: Mass spectrometry (MS): Mass spectrometry is a analytical technique which involves the study of the…

Q: What are the factors which need to be considered in the selection of culture vessel?

A: Culture vessels:- Culture vessels provide a contamination barrier to protect the cultures from the…

Q: elate the terms presented in the first column with the information in the second column; i.…

A: The adhesion of atoms, ions, or molecules from a gas, liquid, or dissolved solid to a surface is…

Q: How to prepare wet mounts and hanging drop mounts of live specimen in microbial motility?

A: Wet mounts and hanging drop mounts of live specimen are used in microbial motility.

Q: Explain in detail the methods that can use to achieve static and or cidal effects in microorganisms

A: Bacterio static effect can be produced by the agents that inhibits the growth of the bacteria. In…

Q: From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking…

A: Counting with the CFU colony-forming units needs culturing the microbes. It counts only the viable…

Q: hich method for introducing microbes on media uses an inoculation loop and the bacterial sample is…

A: Answer. The four-quadrant streak method is used for introducing microbes on media using an…

Q: Explain the importance of using a control tube with only broth when measuring turbidity in a…

A: A spectrophotometer is used to measure the turbidity of a microbial culture sample. It is also used…

Q: the step by step process of Nitrate Redution Test using a schematic diagram

A: Nitrate reduction test This test is widely used in microbiology laboratories to determine the…

Q: I have a culture containing 106 organisms/ml and need to dilute it to 1000 organisms/ ml.   Can…

A: Serial dilution is procedure by which a stock solution containing a high concentration of…

Q: Compare and contrast use dilution and disk diffusion methods of evaluating disinfectants. Please…

A: Chemical disinfectants are gathered by the sorts of organisms and infectious agents they are…

Q: Identify the qualities of enriched, selective, and differential media;use examples to explain their…

A: Microbes are isolated and cultured for various reasons. The role of media is crucial in the culture,…

Q: How is true microbial motility distinguished from other kinds of motion like Brownian movement that…

A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: Describe some of the processes involved in identifying microbesfrom samples.

A: Several tests help in identifying microbes and detecting signs of infection. These techniques could…

Q: Explain the difference between assimilatory nitrate reduction and dissimilatory nitrate reduction.

A: Introduction  Bacteria are single-celled microorganisms that belong to the kingdom Monera. They are…

Q: Explain how to perform a serial dilution

A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…

Q: What additional information can you get by using selective and differential media that you cannot…

A: Media for microorganism culture consists of the substances that are required for the growth of…

Q: what is the proper position for using an incentive spirometer?

A: Lungs are the important organs of the respiratory system allowing filtration of air that is inhaled…

Q: Enlist th five differences between crude media and defined media.

A: Microbiology uses different types of culture media for the growth, culturing, and study of different…