Explain the Part of a human genomic DNA library?
A gene is a unit of hereditary present on the double-helical strands of DNA (deoxyribonucleic acid). The complex strands of DNA are condensed in a form of chromosomes present inside the nucleus of the cells genes carry genetic information regarding genotypic and phenotypic traits and characters. This information is coded in the form of amino acids which are synthesized into functional proteins by translation mechanism. All the encoded message on the DNA is transferable/heritable from the parent to offspring at the time of cell division.
Gene libraries are a collection of genes which are cloned in sufficient number that are likely to contain every single gene present in a particular organism. Genomic libraries are prepared by purifying total cell DNA, and then making a partial restriction digest, resulting in fragments that can be cloned into a suitable vector (shotgun technique). Usually a λ replacement vector, a cosmid, or possibly a yeast artificial chromosome (YAC), bacterial artificial chromosome (BAC) are the choice of vector. YAC vectors are used to clone mammalian genes as, it allows post translational modification and have high copy number unlike, prokaryotic vectors.
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