Explain the importance of maintaining aseptic conditions for the success of the genetic engineering experiment provided below after examining the results.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Explain the importance of maintaining aseptic conditions for the success of the genetic engineering experiment provided below after examining the results.

How the petri dishes for the final incubation were set up:
Both bullet tubes contain
bacteria, but only one tube
receives the plasmid DNA.
PGLO Plasmid DNA
(+PGLO)
(PGLO)
PGLO DNA
PGLGO DNA
After heat-shock and recovery,
the bacteria are plated into 4
dishes of nutrient agar (LB).
Ampicillin (amp) is present in 3
of the dishes, but only one dish
contains arabinose (ara).
*PGLO
*PGLO
*PGLO
"PGLO
The petri dishes are incubated for two days to allow colonies to grow. Because the bacterial
suspensions were spread evenly over the agar, individual colonies can usually be seen; each colony
represents the multiplication of a single bacterium.
LB =Nutrient Agar
LB/AMP= Nutrient Agar plus Ampicillin
(antibiotic)
LB/AMPIARA= Nutrient Agar + Ampicillin
+ Arabinose
DNA
The dishes have been placed under
ultraviolet light in a dark room.
LB/AMP
Note that three of the dishes have
bacterial colonies, but that only one dish
contains fluorescent bacteria.
+ DNA
Explain the results of each petri dish.
LBVAMP
LB/AMPIARA
Transcribed Image Text:How the petri dishes for the final incubation were set up: Both bullet tubes contain bacteria, but only one tube receives the plasmid DNA. PGLO Plasmid DNA (+PGLO) (PGLO) PGLO DNA PGLGO DNA After heat-shock and recovery, the bacteria are plated into 4 dishes of nutrient agar (LB). Ampicillin (amp) is present in 3 of the dishes, but only one dish contains arabinose (ara). *PGLO *PGLO *PGLO "PGLO The petri dishes are incubated for two days to allow colonies to grow. Because the bacterial suspensions were spread evenly over the agar, individual colonies can usually be seen; each colony represents the multiplication of a single bacterium. LB =Nutrient Agar LB/AMP= Nutrient Agar plus Ampicillin (antibiotic) LB/AMPIARA= Nutrient Agar + Ampicillin + Arabinose DNA The dishes have been placed under ultraviolet light in a dark room. LB/AMP Note that three of the dishes have bacterial colonies, but that only one dish contains fluorescent bacteria. + DNA Explain the results of each petri dish. LBVAMP LB/AMPIARA
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