Explain the function of stacking gel, separating gel, and Coomassie blue stain in the electrophoresis experiment.

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: Define fluorescent staining.

A: The advent of microscopy started with the development of optical microscopy. Then the level of…

Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…

A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…

Q: Explain the importance of rinsing with water every after each step in gram staining.

A: Introduction : Bacteria can be categorised in a number of ways depending on their morphology,…

Q: In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name them

A: A type of gel electrophoresis (GE) that is used to quantify and separate desired protein molecules…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a method that diminishes the fluorescence force of an sample. A number of…

Q: How can you account different diffusion rates of three dyes through the agarose media in the Petri…

A: Agar is used as a support medium for bacterial growth and culture in labs.This is extracted from sea…

Q: What is the principle of Grams staining based on the cell wall composition of bacterial cell.

A: Bacteria are a kind of organic cell. They comprise an enormous area of prokaryotic microorganisms.…

Q: Outline the techniques of two-dimensional gel electrophoresisand tandem mass spectroscopy, and…

A: Two-dimensional gel electrophoresis is the primary technique for proteomics work. It separates the…