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Explain how the thin and thick filaments interact in the sliding filament model.
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- ATP within actin subunits is hydrolyzed after assembly into a filament but before disassembly from a filament. in theprocess of assembly into a filament. in the process of disassociation from a filament after disassociation from a filamentExplain the sliding filament mechanismWhat do Intermediate filaments provide?
- If you were to watch muscle tissue contract: Under a light microscope, would you see the muscle fibers get narrower, or the striations get thinner? Explain. At the EM level, focusing on one sarcomere, you would be able to see a region of thick filaments overlapping two regions of thin filaments. Use the structure of the thick filaments to explain how ONE region of thick filaments is able to pull in microfilament in two opposite directions (both toward the center of the sarcomere).Define the types and functions of intermediate filaments.Explain how cofilin distinguish old filaments from new ones.
- How is filament polarity detectable?The ability of myosin to walk along an actin filament may be observed with the aid of an appropriately equipped microscope. Describe how such assays are typically performed. Why is ATP required in these assays? How can such assays be used to determine the direction of myosin movement or the force produced by myosin?Would you expect to find motor proteins that move along intermediate filaments? Explain why or why not.