Answered: Experiment: UV-Vis Spectrophotometry…

Experiment: UV-Vis Spectrophotometry and Beer’s Law Absorption of Light of Dyes in Beverages Explain the purpose of using a blank solution in this experiment.

Living by Chemistry

2nd Edition

ISBN:9781464142314

Author:Angelica M. Stacy

Publisher:Angelica M. Stacy

ChapterU5: Fire: Energy , Thermodynamics, And Oxidation-reduction

SectionU5.116: How Absorbing: Spectroscopy

Problem 6E

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Experiment: UV-Vis Spectrophotometry and Beer’s Law

Absorption of Light of Dyes in Beverages

Explain the purpose of using a blank solution in this experiment.

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Which is TRUE for spectroscopy of a mixture? A. The absorbance for a mixture at a particular wavelength is the sum of the absorbances for the components that absorb at the particular wavelength. B. Spectrophotometers can differentiate between mixture components that absorb at the same wavelength. C. Each component in a mixture has the same molar absorptivity at the same wavelength. D. The concentration for each component in a mixture is easily calculated by least squares for guesses of each component when the individual spectra are well resolved.
For the experimental data used in this lab, why was it okay to use the absorbance values instead of concentrations?
True or False 1. Photodiode arrays are devices within the instrument that help amplify the signal of a sample. 2. Atomic spectroscopy requires the atomization of a sample, which involves nebulization and eventually volatilization to form an elemental vapor. 3. Organic compounds are normally analyzed in atomic spectroscopy despite their small electronic transition range.
Why is it necessary to run a blank before measuring the absorbance of a sample? To allow the lamp and electronics of the spectrophotometer to warm up before taking the sample absorbance reading. So that scattered light will be filtered before hitting the photodetector. To account for the absorbance caused by the cuvette and the solvent To account for the absorbance caused by the cuvette alone .
a. When the light source is monochromatic, Beer's Law holds true. b. Beer's Law establishes a relationship between the concentration of a sample and its absorbance, wherein absorbance is the ratio of light leaving the sample to light entering it. c. For solutions that have more than one absorbing species, Beer's Law holds true even if the analytes of interest don't interact.
Cite one research article that used an IR spectrophotometer in their study and discuss how they used the instrument to generate their data and how they used the data of IR in answering their hypothesis.
Why must solutions with high concentrations be diluted prior to analysis via Beer's Law? A. The relationship between absorbance and concentration is not linear at high concentrations. B. The detector will reach its detection threshold. C. The photon source is too weak to provide accurate results. D. There is no need to work with dilute concentrations; any concentrations will work. E. The molar absorptivity of a compound is dependent on its concentration.
When do you need to blank a spectrophotometer (Spec 20)? After completing all data collection. After the wavelength is changed. Before running a set of samples. Between each sample, even if the wavelength remains the same.
B. Quantitative Spectroscopy Prepare two graphs, %T vs. Concentration and Absorbance vs. Concentration, using the data for the cobalt standards. The concentrations of Cobalt standards 2-4 can be calculated using simple dilution calculations. Concentration should be on the x-axis for each of these graphs. The first should clearly show some curvature while the second should be a linear curve. Using the absorbance graph you can determine the concentration of the unknown. Take the absorbance value for the unknown and find that value on the y-axis. Draw a horizontal line from that point on the y-axis to the plotted line. From the point where the horizontal line intersects the plotted line, draw a vertical line straight down to the x-axis. Record the value from the point where you hit the x-axis as the unknown concentration.
Considering Beer's Law, determine which of the following is TRUE in spectrophotometric analysis. A. Spectrophotometric analysis can only be done in the UV region of the spectrum B. Spectrophotometric analysis requires the use of monochromatic light C. Spectrophotometric analysis must be done using a double beam instrument. D. Spectrophotometric analysis can only be done in the visible region of the spectrum
State whether the statement is correct or incorrect
Chemistry Instrumental Analysis Class The experiment is OES: Cleaning Validation of removal of pharmaceutical drugs and calibration standards by fluoresence spectroscopy - Why the instrument use two detectors and monochromators? What type of monochromators are used in our instrument? - Do you think that a similar experiment can be prepared for the analysis of naproxen and benzoic acid? Explain.