Experiment: DNA Extraction from Banana The procedures are attached below. Questions: 1. What does mashing do to the fruit? And why is it needed to add detergents? 2. What do you think the ethanol does? Why can’t we use room temperature ethanol?
Experiment: DNA Extraction from Banana The procedures are attached below. Questions: 1. What does mashing do to the fruit? And why is it needed to add detergents? 2. What do you think the ethanol does? Why can’t we use room temperature ethanol?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Experiment: DNA Extraction from Banana
The procedures are attached below.
Questions:
1. What does mashing do to the fruit? And why is it needed to add detergents?
2. What do you think the ethanol does? Why can’t we use room temperature ethanol?
Transcribed Image Text:B. DNA Extraction from Banana
1. Put the ½ banana and ¼ cup distilled water in the sealable plastic bag and mash to make your
slurry.
2. In a beaker, mix a solution of 1 tsp. shampoo, 2 pinches of salt and 4 tsp. distilled water. Stir
this solution slowly for about a minute until the shampoo dissolves in the water.
Add 2 tsp. of the banana slurry to the soap solution and stir for 5 minutes.
Make a filtration set-up and pour in the liquid mixture and let it be filtered. Set aside.
Fill a test tube with 2 tsp. of cold ethanol. Very slowly, add 2 tsp. of the filtered banana mixture
to make two layers of liquid.
6. Let the test tube sit for 2-3 minutes without disturbing the solution. You will see the clear/white
DNA precipitate into the alcohol layer.
7. Keep the DNA, remove it using a bamboo skewer (a twirling motion works best) and place it in
a capped tube filled with alcohol. (Optional)
3.
4.
5.
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