Expansion of a CAG repeat region by 1 repeat is an example of a frameshift mutation. Both DNA polymerase and RNA polymerase read their template strand in a 3'->5' direction. A silent mutation can create a RFLP.
Q: The following sequence of nucleotides is found in a single-stranded DNA…
A: DNA is the double-stranded molecule that is the genetic material in most animals except for some…
Q: . The table opposite shows the standard (coding strand) DNA rinlet codes for the 20 amino acids…
A: Introduction :- The process of protein synthesis occurs in two steps which are transcription and…
Q: a. Indicate whether point C is a 5' end or a 3' end of a nucleic acid. b. Indicate which strand…
A: The ribonucleic acid was the primary genetic material. It acts as a genetic material furthermore as…
Q: In the diagram shown below representing a replisome, the portion represented by the letter functions…
A: Ans. The method of DNA replication is a process in which double-strand DNA molecule copying in order…
Q: DNA primase synthesizes a short RNA primer that later appears at the 5'-end of each Okazaki…
A: The given statement is TRUE
Q: A researcher was mutating prokaryotic cells by inserting segments of DNA. In this way, she made the…
A: a.) Mutant is the palindromic sequence for original construct. Palindromic sequences are those…
Q: Transposons are useful as mutagens because they act asmolecular tags for genomic DNA sequences that…
A: Step 1 Transposons or jumping gene is a DNA (deoxyribonucleic acid) sequence that can change its…
Q: The following sequence of nucleotides is found in a single-stranded DNA template:…
A: The process of formation of mRNA from DNA is called transcription.
Q: Consider which of the five mutations is most likely to cause Duchenne muscular dystrophy in this…
A: Duchenne muscular dystrophy is a disease charecterised by muscular weakness due to lack of a protein…
Q: What is a promoter? What is the function of RNA polymerase? How does it differ from DNA polymerase?
A: In the process of transcription, there are several machinery and enzymes involved. This aids in the…
Q: How would nucleotide excision repair be affected if one of the followingproteins was missing?…
A: The process of identification and correction of damaged DNA molecule by a cell which encodes its…
Q: In reverse transcriptase polymerase chain reaction (RT-PCR), an analytical method used to amplify…
A: Reverse transcriptase polymerase chain reaction (RT-PCR) is a method used to amplify and sequence…
Q: In relation to central dogma of molecular biology answer the following questions: The following…
A: Central dogma of molecular biology depicts how information in the DNA is transcribed into RNA and…
Q: Scientists developed a marker technology to examine the function of RNA polymerase in yeast cells.…
A: Transcription process is responsible for producing mRNA. It takes place in the nucleus.
Q: explain strand-slippage replication for STR mutation
A: Kornberg et al. proposed the idea in 1964, and it is also known as DNA slippage, polymerase…
Q: An adult with a history of tanning has his genome sequenced. The beginning of a protein-coding…
A: The main inheritable material is Deoxyribonucleic acid (DNA). It is composed of four nitrogenous…
Q: A particular variant of the lambda bacteriophage has a DNA double-stranded genome of 51,365 base…
A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…
Q: Process by which the DNA sequences encoding exons are exchanged and reordered through genetic…
A: Exon :- the coding part of the gene. Intron :- the non coding regions of pre mature mRNa.
Q: rmal hemoglobin is created from the codon GAA, which codes for glutamic d while sickle-cell…
A: Any abrupt change in the DNA sequence of nucleotides results in mutation its effects may be diverse…
Q: From standpoint of replication and transcription, explain how RNA polymerase is allowed to…
A: DNA is a double standard molecule. RNA is a single standard molecule. RNA is formed from DNA…
Q: . Locate the -10 region hexanucleotide sequence in the following coding strand of DNA. Indicate…
A: Transcription is a process through which the template strand of the DNA transcribes into mRNA in the…
Q: Is each of the following mutations a transition, transversion, addition,or deletion? The original…
A: Since no specific subparts have been asked to be answered, the first three subparts have been…
Q: Shown below is a drawing showing the result of an experiment in which an RNA molecule is allowed to…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: In which region(s) will Okazaki fragments be found during new strand synthesis? (see image for…
A: Semi replicative mechanism is a type of DNA mechanism which explained the type of replication…
Q: Which of the following best describes this type of mutation? Original – CCU-GAU-GAG-UCA…
A: Introduction : Mutations are modifications to the genetic sequence. Mutations can involve the…
Q: What type of mutation (transition, transversion, or frameshift)would you expect each of the…
A: Mutagens are such agent that changes the genetic material, that is, the structure of DNA…
Q: DNA is made of two strands that are antiparallel. If one strand runs from 3’ to 5’ direction the…
A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…
Q: Addition or deletion of bases causes which kind of mutation? Select one: O a. Transversion O b. •…
A: Any heritable change that happens in the nucleotide sequence of the DNA is called a mutation.…
Q: Taq polymerase is a bacterial thermostable DNA polymerase that has a relatively low replication…
A: Taq polymerase is an enzyme used to amplify DNA in Polymerase chain reactions. Taq polymerase is a…
Q: DNA repair systems are responsible for maintaining genomic fidelity in normal cells despite the high…
A: Living organisms are constantly exposed to a variety of DNA-damaging substances that can have an…
Q: A mistake during DNA replication leads to a mutation in the nucleotide sequence shown below. DNA DNA…
A: Mutations are a genetic phenomenon in which alterations in the sequence of nucleotides of DNA occur.…
Q: RNA polymerase from E. coli (core enzyme alone) has all of the following properties except:…
A: RNA polymerase enzyme is a multi subunit enzyme having a catalytically active core.
Q: Several temperature-sensitive mutant strains of E. coli displaythe following characteristics.…
A: D.N.A. bio synthesis -- The synthesis of D.N.A. occurs when a cell divides and during division of…
Q: Faulty DNA repair; ii) Gain-of-function; and iii) Trinucleotide repeats provide genetics disorders…
A: Asked : Example of mutation are asked with disorder for given condition.
Q: Select the statement(s) that accurately describe the function of DNA polymerase and the types of…
A: Mutation is a phenomenon that results in alteration of DNA sequences. This results in changes in the…
Q: A mutation in which of these proteins will lead to increased mutations in all daughter cells? A.…
A: The mutation is a heritable change that arises due to the alteration of the genomic sequence of an…
Q: Below are shown two views of the backbone representation of the Myc-Max complex binding to DNA (PDB…
A: DNA ( Deoxyribonucleic acid) is the hereditary molecule of organism as it flows from one generation…
Q: Compare DNA polymerase and RNA polymerase from E. coli in regard to each of the following features:…
A: Introduction: DNA polymerases are the group of enzymes that catalyze the synthesis of DNA strands…
Q: Huntington’s disease is an inherited neurological ailment with a variable age of onset. A protein…
A: Huntington's disease is a neurodegenerative disorder. The repetition of a CAG trinucleotide causes…
Q: an adult with a history of tanning has his genome sequenced. The beginning of a protein coding…
A: Mutations are the changes in the genetic sequence that may result in genotypic or phenotypic…
Q: Evaluate the mutation below. Original DNA strand: 3'-TACTTACGCACGGCCACT-5' Amino acids produced:…
A: From the DNA sequence the mRNA is produced within the nucleus of the cell by the transcription…
Q: Original DNA template: 3'-ACGGTCAATTTGCTG-5 a) Transcribe the sequence. b) Translate the sequence.…
A: “Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…
Q: Both function as holoenzymes that have polymerase and helicase activities Both bind promoters Both…
A: Characteristics shared by both RNA polymerase and DNA pol III in E.coli Statement A ) RNA Polymerase…
Q: A mutant DNA strand was transcribed then translated to proteins. a. What is the protein product of…
A: A mutation is an abnormal change in DNA sequences that alters the protein product and is one of the…
Q: Explain why the active site of poly(A) polymerase is much narrower than that of DNA and RNA…
A: Poly (A) polymerase is the enzyme that is an important part of the polyadenylation machinery. It is…
Give answer in:
TRUE OR FALSE
Step by step
Solved in 2 steps
- Knock out mice that are mutant for a gene product X die. Variants of the X gene that were reintroduced through homologous recombination indicate that variant proteins that lack the C-terminal region of the protein cannot rescue the lethality (did not allow them to live), and always localized to the cytoplasm. Those that retained the C-terminal region rescued the lethality (conferred viability!) and were consistently localized within the nucleus. RNA-seq analysis of the mutant cells vs the wild-type cells indicated that the expression of many genes that are essential for neural function was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved between mouse and humans at both the amino acid and the nucleotide level. The predicted mouse mRNA sequence is shown below where the AUG corresponds to the translational start site (AUG). 5’-AUGUUUACAGAGGGGAAU... -3’ d) What motif could be present to direct this protein to its correct destination? e)…Knock out mice that are mutant for a gene product X die. Variants of the X gene that were reintroduced through homologous recombination indicate that variant proteins that lack the C-terminal region of the protein cannot rescue the lethality (did not allow them to live), and always localized to the cytoplasm. Those that retained the C-terminal region rescued the lethality (conferred viability!) and were consistently localized within the nucleus. RNA-seq analysis of the mutant cells vs the wild-type cells indicated that the expression of many genes that are essential for neural function was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved between mouse and humans at both the amino acid and the nucleotide level. The predicted mouse mRNA sequence is shown below where the AUG corresponds to the translational start site (AUG). 5’-AUGUUUACAGAGGGGAAU... -3’ Q3a) Using a total RNA sample obtained from human cells, what is the first primer you would…People with a commonly occurring, wild type allele of PTC with two adjacent thymines at a particular site in the coding sequence are more prone to BCCs than people without this allele. How can this be explained (one sentence)? The "two adjacent thymines" allele of PTC causes a bigger increase in BCC risk for people xeroderma pigmentosum (XP), who lacks components of the nucleotide excision repair pathway, compared to people without XP. How can this be explained (one sentence)?
- Translesion synthesis (TLS) polymerase zeta (ζ) is capable of accurately reading through 6,4-photoproducts (cytotoxic damage). If this protein is non-functional: a different TLS polymerase will accurately read through the DNA lesion. a different TLS polymerase will inaccurately read through the DNA lesion resulting in increased mutational frequency. the replicative polymerase will inaccurately read through the DNA damage. DNA polymerase alpha (α) will be recruited to synthesize a primer across the DNA damage. the DNA lesion cannot be bypassed and will result in double stranded DNA breaks.(i) For the chromatogram below, what is the sequence of the template DNA from base 115 to 125? CTGTGTGAAATTGT TA T CCGC T CA CA AT T C CACA CA A CATA CGAGC CGGAAG CA TA A 110 120 130 140 150 160 (ii) An allele of a gene has the following change in it's sequence ATG GTG CÁC CTG ACT CCT GTG GAG AAG TCT compared to the wild type ATG GTG CAC CTG ACT CT GAG GAG AAG TCT With reference to the sequence; there is a codon, resulting in a change from is a mutation in the to which mutation.V/D/J recombination frequently yields a gene whose mRNA cannot be successfully translated into an immunoglobulin chain. What aspect of somatic recombination is likely to produce nonproductive gene rearrangement?
- A certain section of the coding (sense) strand of some DNA looks like this: 5'- ATGGGCCACTCATCTTAG-3' It's known that a very small gene is contained in this section. Classify each of the possible mutations of this DNA shown in the table below. I Don't Know mutant DNA 5'- ATG GGCCACAGTTCTTAG-3' 5'- ATG GG CTCATCTTAG - 3' 5'- ATG GGCCACGCATCTTAG-3' Submit type of mutation (check all that apply) ооооо O point O silent O noisy ооооо insertion deletion insertion O deletion Opoint Osilent noisy insertion O deletion ооооо Opoint silent O noisy X S Ⓒ2023 McGraw Hill LLC. All Rights Reserved. Terms of Use | Privacy Center AccessibilityYou have the following DNA coding sequence of a wild-type allele: 5’-ATG TTC CAG CTA GAT GAT ATG CTG GTA ATT GGG GAA CGC GCG CGG TAA-3’ For each of the following mutations: A. State whether the mutation is missense, nonsense, frameshift, or silent. B. Write the codon change that occurs for the missense, nonsense, and silent mutations (ex. GAA -- GAT). C. For frameshift mutations, write out the entire mutant sequence with each codon clearly indicated (if the frameshift creates a new stop codon, end the sequence at the new stop). Using the wild type DNA sequence above as a guide : Write the amino acid sequence of the mutants. Mutant 1: transition at nucleotide 23 Mutant 2: T --> G transversion at nucleotide 29 Mutant 3: an insertion of “A” after nucleotide 14 Mutant 4: transition at nucleotide 7 Mutant 5: An insertion of GG after nucleotide 40 Mutant 6: transition at nucleotide 15 Mutant 7: a deletion of nucleotide 25You have a patient in your clinic presenting symptoms of cystic fibrosis. You screen their CFTR gene for mutations, and find the following list: CFTR 320 L V CFTR 341 S W CFTR 528 E D CFTR 976 F Q CFTR 1235 S R Which mutation(s) are likely causing cystic fibrosis in this patient? You also sequence a newborn family member of this patient. They have all of these same mutations, other than the one at position 976, and no other mutations in CFTR. Do you predict this person will develop cystic fibrosis? Explain why.
- Exposure to various chemicals can cause DNA mutations. Classify the examples as representative of transition, transversion, or nsertion/deletion mutations. Transition replacement of T:A: with C:G treatment with nitrogen mustard causes this type of mutation oxidative deamination via nitrous acid causes this type of mutation treatment with 5-bromouracil causes the replacement of A:T with G:C Transversion replacement of G:C with T:A Answer Bank Insertion/Deletion the removal of one or more base pairs treatment with proflavin causes this type of mutationBelow is a portion of an exon from a gene that encodes protein X in the genome of the plant Arabidopsis. Wildtype DNA3’ TTC AAT GCT CCG AAT ACC 5’ template strand5’ AAG TTA CGA GGC TTA TGG 3’ non-template strand A new strain (Strain B) of Arabidopsis is identified with the same region of the gene coding for protein X: 3’ TTC AAT GCT CCC AAT ACC 5’ template strand5’ AAG TTA CGA GGG TTA TGG 3’ non-template strand Compare the two DNA sequences and look for any differences. Based on what you find a. There is no mutation in Strain B compared to Strain A. b. After the point of the mutation, all the amino acids encoded by the Strain B template will be different than the Strain A protein X. c. Protein X made from the Strain B template will be much shorter than protein X made from the Strain A template d. Protein X from Strain B will have one amino acid difference that would not affect protein function. e. There is a mutation but there will not be any difference in the…Different Hfr strains have the F factor DNA integrated into their chromosome at different locations due to Different Hfr strains have the F factor DNA integrated into their chromosome at different locations due to homologous recombination between the F factor's origin of replication and the bacterial chromosome's origin of replication. homologous recombination between an IS element within the F factor and an IS element that may be located at different chromosomal locations in different E. coli strains. random breaks that occur within the bacterial chromosome. recombination between homologous chromosomal regions of donor and recipient cells during conjugation.