Exactly 10.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000μg/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, 2.00 mL. The fluorescence of each of these solutions was measured with a fluorimeter, which gave values of 3.26, 4.80, 6.41, 8.02, 9.56, respectively.a. plot the data. b. derive a least squares equation for the data plotted in (a).c. find the concentration of phenobarbital from the equation in (b).d. calculate a standard deviation for the concentration obtained in (c).

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Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Exactly 10.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000μg/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, 2.00 mL. The fluorescence of each of these solutions was measured with a fluorimeter, which gave values of 3.26, 4.80, 6.41, 8.02, 9.56, respectively.

a. plot the data.

b. derive a least squares equation for the data plotted in (a).

c. find the concentration of phenobarbital from the equation in (b).

d. calculate a standard deviation for the concentration obtained in (c).

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