Enzymes are proteins that speed up chemical reactions. Scientists are always looking for efficient and cost saving sources of enzymes that can be used for industrial purposes. Industries often screen organisms for their enzymatic activity in an effort to find useful enzymes. The table below illustrates two fungi and their novel enzymes that have numerous applications in paper, pulp and textile industries

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Enzymes are proteins that speed up chemical reactions. Scientists are always looking for efficient and
cost saving sources of enzymes that can be used for industrial purposes. Industries often screen
organisms for their enzymatic activity in an effort to find useful enzymes. The table below illustrates
two fungi and their novel enzymes that have numerous applications in paper, pulp and textile
industries.
Fungus
Enzyme
Function
Breaks down complex carbohydrates
called xylans into individual sugars
called xylose.
Breaks down starch into individual
Leptosphaerulina chartanum
Xylanolyase (Enzyme X)
Penicilium oxalicum
Glucoamylase (Enzyme G)
sugars of glucose.
(Raferance: Xu et al. Biotechnol Biotueis (2016) 9:216 and Wu et al. PLOS One (2013) a (9
Enzymes X and G were isolated from their respective fungi and their activity was measured at various
pH to determine which pH conditions were ideal for industrial applications. The data is shown in the
table below.
1. Draw a line graph to represent the data. Compare the two
enzymes.
% Activity of % Activity of
Xylanolyase Glucoamylase
(Enzyme X)
pH
(Enzyme G)
3
34
70
4
46
92
98
85
6
41
75
22
65
2. If the industry wanted to use both enzymes under one pH condition which pH should they adjust
their production system to so that they can maximize the action of both enzymes?
1
Transcribed Image Text:Enzymes are proteins that speed up chemical reactions. Scientists are always looking for efficient and cost saving sources of enzymes that can be used for industrial purposes. Industries often screen organisms for their enzymatic activity in an effort to find useful enzymes. The table below illustrates two fungi and their novel enzymes that have numerous applications in paper, pulp and textile industries. Fungus Enzyme Function Breaks down complex carbohydrates called xylans into individual sugars called xylose. Breaks down starch into individual Leptosphaerulina chartanum Xylanolyase (Enzyme X) Penicilium oxalicum Glucoamylase (Enzyme G) sugars of glucose. (Raferance: Xu et al. Biotechnol Biotueis (2016) 9:216 and Wu et al. PLOS One (2013) a (9 Enzymes X and G were isolated from their respective fungi and their activity was measured at various pH to determine which pH conditions were ideal for industrial applications. The data is shown in the table below. 1. Draw a line graph to represent the data. Compare the two enzymes. % Activity of % Activity of Xylanolyase Glucoamylase (Enzyme X) pH (Enzyme G) 3 34 70 4 46 92 98 85 6 41 75 22 65 2. If the industry wanted to use both enzymes under one pH condition which pH should they adjust their production system to so that they can maximize the action of both enzymes? 1
3. Proteins are important for numerous functions in a cell and their activity/function is dependent on
their structure. Each protein folds into its respective structure and ionic bonds between lons in side
chains of proteins drives protein folding as shown by the oppositely charged ions in the figure below.
Protein
• Draw a picture similar to the one shown above to show what will happen to such interactions when
the pH increases. Show how this impacts protein structure. (Hint: Is the hydrogen lon
concentration high or low? Note that hydrogen ions can be added or lost into molecules depending
on the pH and that the NH, is affected in this case.)
• Draw a picture similar to the one shown above to show what will happen to such interactions when
the pH decreases. Show how this impacts protein structure, (Hint: is the hydrogen ion
concentration high or low? Note that hydrogen ions can be added or lost into molecules depending
on the pH and that the COO is affected in this case.)
• What other type of bond can impact protein folding?
4. Look back at the graph you drew for Q1 and based on your answers for Q3 state how enzyme X
and enzyme Gare different from each other such that they are differently impacted by pH.
Transcribed Image Text:3. Proteins are important for numerous functions in a cell and their activity/function is dependent on their structure. Each protein folds into its respective structure and ionic bonds between lons in side chains of proteins drives protein folding as shown by the oppositely charged ions in the figure below. Protein • Draw a picture similar to the one shown above to show what will happen to such interactions when the pH increases. Show how this impacts protein structure. (Hint: Is the hydrogen lon concentration high or low? Note that hydrogen ions can be added or lost into molecules depending on the pH and that the NH, is affected in this case.) • Draw a picture similar to the one shown above to show what will happen to such interactions when the pH decreases. Show how this impacts protein structure, (Hint: is the hydrogen ion concentration high or low? Note that hydrogen ions can be added or lost into molecules depending on the pH and that the COO is affected in this case.) • What other type of bond can impact protein folding? 4. Look back at the graph you drew for Q1 and based on your answers for Q3 state how enzyme X and enzyme Gare different from each other such that they are differently impacted by pH.
Expert Solution
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  • Proteins are important for numerous functions in a cell and their activity is dependent on their structure.

 

  • The pH also impacts the protein structure.
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