Enzyme activity is an important characteristic of enzymes, and its units are moles (M) or milimoles (mM) of substrate produced in a given unit of time (e.g. 1 second). The table below contains data from an analysis of enzyme 1. In this experiment, replicate samples contained the same amount of enzyme, and were supplied with different amounts of substrate. After 10 minutes, the amount of product that had been produced was measured. The equation for enzyme kinetics is shown below to help you think about the reactions at each stage. E+SESE+P 1. Part 1. Graph the activity of enzyme 1, label the X and Y axes, and then answer questions 1-5. (Hint: Identify the dependent variable. Put that on the Y axis) 100 90 80 70 60 50 40 30 20 10 Ō 10 20 30 40 50 60 70 80 90 10⁰ 2. How did enzyme activity respond when the substrate concentration ranged from 0 to 20 mM? 2. Why did enzyme activity respond in this way? 3. How does enzyme activity respond to substrate concentrations from 50 to 100 mM? 4. Why does enzyme activity respond in this way? Enzyme 1 substrate product conc. made in (MM) 10 min. 0 10 20 30 40 50 60 70 80 90 100 0 75 88 90 90.5 89.5 91 89.8 89 90 90

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Enzyme activity is an important characteristic of enzymes, and its units are moles (M) or milimoles (mM) of
substrate produced in a given unit of time (e.g. 1 second). The table below contains data from an analysis of
enzyme 1. In this experiment, replicate samples contained the same amount of enzyme, and were supplied
with different amounts of substrate. After 10 minutes, the amount of product that had been produced was
measured. The equation for enzyme kinetics is shown below to help you think about the reactions at each
stage.
E+SESE+P
1. Part 1. Graph the activity of enzyme 1, label the X and Y axes, and then answer
questions 1-5. (Hint: Identify the dependent variable. Put that on the Y axis)
100
90
80
70
60
50
40
& M
30
20
10
O 10 20 30 40 50 60 70 80 90 100
2. How did enzyme activity respond when the substrate concentration ranged
from 0 to 20 mM?
2. Why did enzyme activity respond in this way?
3. How does enzyme activity respond to substrate concentrations from 50 to 100 mM?
4. Why does enzyme activity respond in this way?
Enzyme 1
substrate product
conc.
made in
(MM)
10 min.
10
20
30
40
50
60
70
80
100
0
75
88
90
90.5
89.5
91
89.8
89
90
90
Transcribed Image Text:Enzyme activity is an important characteristic of enzymes, and its units are moles (M) or milimoles (mM) of substrate produced in a given unit of time (e.g. 1 second). The table below contains data from an analysis of enzyme 1. In this experiment, replicate samples contained the same amount of enzyme, and were supplied with different amounts of substrate. After 10 minutes, the amount of product that had been produced was measured. The equation for enzyme kinetics is shown below to help you think about the reactions at each stage. E+SESE+P 1. Part 1. Graph the activity of enzyme 1, label the X and Y axes, and then answer questions 1-5. (Hint: Identify the dependent variable. Put that on the Y axis) 100 90 80 70 60 50 40 & M 30 20 10 O 10 20 30 40 50 60 70 80 90 100 2. How did enzyme activity respond when the substrate concentration ranged from 0 to 20 mM? 2. Why did enzyme activity respond in this way? 3. How does enzyme activity respond to substrate concentrations from 50 to 100 mM? 4. Why does enzyme activity respond in this way? Enzyme 1 substrate product conc. made in (MM) 10 min. 10 20 30 40 50 60 70 80 100 0 75 88 90 90.5 89.5 91 89.8 89 90 90
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