E. coli BL21 cells carrying a pQE expression vector can be induced to produce large amounts of a protein of interest by adding which of the following compounds to the culture medium:

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**Title:** Induction of Protein Expression in *E. coli* BL21 Cells

**Topic:** Protein Expression Induction Methods

**Question:**

_Escherichia coli_ (E. coli) BL21 cells carrying a pQE expression vector can be induced to produce large amounts of a protein of interest by adding which of the following compounds to the culture medium:

- a. ampicillin
- b. X-gal
- c. Tris
- d. isopropyl beta-D-1-thiogalactopyranoside (IPTG)
- e. ethylenediaminetetraacetic acid (EDTA)
- f. glucose
- g. imidazole

**Explanation:**

The correct answer is **d. isopropyl beta-D-1-thiogalactopyranoside (IPTG)**. IPTG is commonly used as an inducer for systems requiring the expression of proteins in E. coli. It mimics allolactose, a lactose metabolite that triggers the transcription of genes downstream of the lac operator in the lac operon. By using IPTG in a pQE expression vector system, protein production is initiated efficiently, without being metabolized by the bacteria, leading to the production of the protein of interest.
Transcribed Image Text:**Title:** Induction of Protein Expression in *E. coli* BL21 Cells **Topic:** Protein Expression Induction Methods **Question:** _Escherichia coli_ (E. coli) BL21 cells carrying a pQE expression vector can be induced to produce large amounts of a protein of interest by adding which of the following compounds to the culture medium: - a. ampicillin - b. X-gal - c. Tris - d. isopropyl beta-D-1-thiogalactopyranoside (IPTG) - e. ethylenediaminetetraacetic acid (EDTA) - f. glucose - g. imidazole **Explanation:** The correct answer is **d. isopropyl beta-D-1-thiogalactopyranoside (IPTG)**. IPTG is commonly used as an inducer for systems requiring the expression of proteins in E. coli. It mimics allolactose, a lactose metabolite that triggers the transcription of genes downstream of the lac operator in the lac operon. By using IPTG in a pQE expression vector system, protein production is initiated efficiently, without being metabolized by the bacteria, leading to the production of the protein of interest.
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