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Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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PROBLEM t! Bcl-X, is an anti-apoptotic protein, which is upregulated in cancer cells. Bcl-X activity can be inhibited by peptides derived from proapoptotic proteins such Bak, Bad, and Bax. Fluorescence polarization has been used for determining their inhibition capacity. Below you find the semilog plot for the direct binding experiment, in which frepresents fluorescein. 300 -bax O f-bak 200- v - bad 6 100- 10 10 10 10 10 10 10 10 Log (Bet-x, M) 4.1 From the plot, estimate the dissociation constant. Please, report your answer as nM. Using arrows or dashed lines indicate the mP value where f-bad approaches 100% bound and 100 % unbound 4.2 Suggest reaction conditions to conjugate fluokescein Comment where will you introduce the fluorophore. With which approach could you enhance the apoptosis effect further? 4-3 to the peptides. 4-4 mP
Transcribed Image Text:PROBLEM t! Bcl-X, is an anti-apoptotic protein, which is upregulated in cancer cells. Bcl-X activity can be inhibited by peptides derived from proapoptotic proteins such Bak, Bad, and Bax. Fluorescence polarization has been used for determining their inhibition capacity. Below you find the semilog plot for the direct binding experiment, in which frepresents fluorescein. 300 -bax O f-bak 200- v - bad 6 100- 10 10 10 10 10 10 10 10 Log (Bet-x, M) 4.1 From the plot, estimate the dissociation constant. Please, report your answer as nM. Using arrows or dashed lines indicate the mP value where f-bad approaches 100% bound and 100 % unbound 4.2 Suggest reaction conditions to conjugate fluokescein Comment where will you introduce the fluorophore. With which approach could you enhance the apoptosis effect further? 4-3 to the peptides. 4-4 mP
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