During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification

Q: Use the figure of the gel below to answer the following questions. Controls 1 2 3 Experimentals 4 5…

A: Gel electrophoresis is a technique used to separate DNA fragments,( or may be RNA and protein) based…

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: Disorders associated with and epigenetic defect resulting in decreased protein expression should be…

A: The use of molecular tests for diagnosing disorders associated with epigenetic defects resulting in…

Q: The PCR technique requires a DNA polymerase from an organism that can endure high heat, such as…

A: PCR is referred to as polymerase chain reaction. It was discovered by a biochemist Kary Mullis. It…

Q: When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose…

A: The objective of the question is to understand the purpose of DNA-marker molecules in a gel…

Q: Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the…

A:

Q: When the SEBI gene is inserted with a defective leading to phenotype. bacterial DNA, wrinkle…

A: Phenotype refers to the observable physical or biochemical characteristics of an organism resulting…

Q: Why can we use temperature sensitive genetic screening to identify particular mutants within a…

A: Temperature-sensitive (TS) mutations are rare alleles that are used to study the expression of…

Q: Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to…

A: PCR stands for a polymerase chain reaction. It is used to amplify the DNA segment. It can make many…

Q: Pcr primers are:  single strand 15-25 bases long incorporated into the newly synthesized DNA…

A: PCR stands for polymerase chain reaction. It is one of the technique of biotechnology.

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…

A: PCR stands for polymerase chain reaction.

Q: ** Application of Abs in diagnosis and research: What is to be measured - Ag or Ab? Is the assay…

A: Since you have uploaded multiple questions, only first three subparts have been answered as per…

Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…

A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…

Q: Could you please explain these:   1. Why analyze the unknowns in a (short) serial dilution rather…

A: Introduction: DNA concentration refers to the amount of DNA present in a sample. It is typically…

Q: Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.

A: Main components for preparation of cloning vector are :  Origin of replication Marker gene…

Q: Look over the protocol for the PTC lab. If the tube containing the DNA and HaeIII were NOT incubated…

A: HaeIII is a type II restriction endonuclease enzyme that recognizes and cleaves DNA at a specific…

Q: ELISA is a vital diagnostic tool. Reagents required for this technique include Thermostable DNA…

A: ELISA (Enzyme-linked immunosorbent assay) is a sensitive and specific laboratory technique used to…

Q: If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be…

A: Dye based assay is a dye binding protein assay based on the binding of protein molecules to…