Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.8% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 125 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals) **Assume the dissolution of agarose will not alter the final volume of the solution** Polymerase Chain Reaction (PCR) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.029 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 4.6 nanograms per microliter? Report your answer to two decimal places using normal decimal notation (e.g. 0.5, not 5.0e-1)
Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.8% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 125 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals) **Assume the dissolution of agarose will not alter the final volume of the solution** Polymerase Chain Reaction (PCR) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.029 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 4.6 nanograms per microliter? Report your answer to two decimal places using normal decimal notation (e.g. 0.5, not 5.0e-1)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA
out on a gel to separate the fragments based on size. To do this she must prepare a 1.8% agarose gel, where the
percentage is determined as mass/vol.
How many grams of agarose must she add to 125 mL of buffer in order to arrive at the correct percentage? (answer
to 2 decimals)
**Assume the dissolution of agarose will not alter the final volume of the solution**
Polymerase Chain Reaction (PCR) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme
from Thermophilus aquaticus to amplify DNA extracted from any sample. In order for the reaction to work, researchers
must transfer microliters of sample to the reaction vessel for PCR.
If a researcher has a DNA sample with a concentration of 0.029 micrograms per microliter, how many microliters of
DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 4.6 nanograms per
microliter?
Report your answer to two decimal places using normal decimal notation (e.g. 0.5, not 5.0e-1)
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