Doug Schemske is a biologist who studies plants from around the world.  Doug and his research team carry out experiments with the plant species Mouse-ear Cress, or Arabidopsis thaliana. They like this species because it is easy to grow in both the lab and field. Arabidopsis is very small and lives for just one year. It grows across most of the globe and in a wide range of latitudes and climates. Arabidopsis is also able to pollinate itself and produce many seeds, making it possible for researchers to grow many individuals to use in their experiments.  Doug chose Arabidopsis populations in Scandinavia and the Mediterranean for his research on local adaptation because those two locations have very different climates. The populations may have adapted to have the highest survival and reproduction based on the climate of their home location.  To deal with sudden freezes and cold winters in Scandinavia, plants may have evolved freeze tolerance traits, which produces chemicals that act like antifreeze. These chemicals accumulate in their tissues to keep the water from turning into ice and forming crystals. To see whether freeze tolerance was driving local adaptation, Doug set up an experiment to identify which plants survived after freezing. Doug collected seeds from several different populations across Scandinavia and the Mediterranean.  He chose locations that had different latitudes because latitude affects how cold an area gets over the year. High latitudes (Scandinavia) are generally colder and low latitudes (Mediterranean) are generally warmer. Doug grew more seedlings for this experiment, and then, when they were a few days old, he put them in a freezer. Doug counted how many seedlings froze to death, and how many survived, and he used these numbers to calculate the percent survival for each population. To gain confidence in his results, he did this experiment with three replicate genotypes per population.     Doug noticed the INNF Scandinavian plant populations had the highest rate of survival in the deep freezer.  Explain how the genetic information in the INNF populations increased their rate of survival in Scandinavia.  Discuss the factors that cause populations to change over time.

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Doug Schemske is a biologist who studies plants from around the world.  Doug and his research team carry out experiments with the plant species Mouse-ear Cress, or Arabidopsis thaliana. They like this species because it is easy to grow in both the lab and field. Arabidopsis is very small and lives for just one year. It grows across most of the globe and in a wide range of latitudes and climates. Arabidopsis is also able to pollinate itself and produce many seeds, making it possible for researchers to grow many individuals to use in their experiments. 

Doug chose Arabidopsis populations in Scandinavia and the Mediterranean for his research on local adaptation because those two locations have very different climates. The populations may have adapted to have the highest survival and reproduction based on the climate of their home location. 

To deal with sudden freezes and cold winters in Scandinavia, plants may have evolved freeze tolerance traits, which produces chemicals that act like antifreeze. These chemicals accumulate in their tissues to keep the water from turning into ice and forming crystals. To see whether freeze tolerance was driving local adaptation, Doug set up an experiment to identify which plants survived after freezing. Doug collected seeds from several different populations across Scandinavia and the Mediterranean. 

He chose locations that had different latitudes because latitude affects how cold an area gets over the year. High latitudes (Scandinavia) are generally colder and low latitudes (Mediterranean) are generally warmer. Doug grew more seedlings for this experiment, and then, when they were a few days old, he put them in a freezer. Doug counted how many seedlings froze to death, and how many survived, and he used these numbers to calculate the percent survival for each population. To gain confidence in his results, he did this experiment with three replicate genotypes per population.

 

 

Doug noticed the INNF Scandinavian plant populations had the highest rate of survival in the deep freezer.  Explain how the genetic information in the INNF populations increased their rate of survival in Scandinavia.  Discuss the factors that cause populations to change over time. 

 

Average Survival After Freezing (%)
100
90
80
70
60
50
40
30
20
10
68.33
26.00
0
Scandinavia
Mediterranean
Source of Seeds
Figure 1. Average survival (+/- 1 standard error) of Arabidopsis thaliana seedlings after freezing.
Seeds were from 4 different sites in Scandinavia and 4 different sites in the Mediterranean. Three
trails were conducted with seeds from each site.
Transcribed Image Text:Average Survival After Freezing (%) 100 90 80 70 60 50 40 30 20 10 68.33 26.00 0 Scandinavia Mediterranean Source of Seeds Figure 1. Average survival (+/- 1 standard error) of Arabidopsis thaliana seedlings after freezing. Seeds were from 4 different sites in Scandinavia and 4 different sites in the Mediterranean. Three trails were conducted with seeds from each site.
Source of
Seeds
Trial #
1
Scandinavia
Scandinavia
2
Scandinavia
3
Scandinavia
1
Scandinavia
2
Scandinavia
3
Scandinavia
1
Scandinavia
2
Scandinavia
3
Scandinavia
1
Scandinavia
2
Scandinavia
3
Mediterranean 1
Mediterranean 2
Mediterranean 3
Mediterranean 1
Mediterranean 2
Mediterranean 3
Mediterranean 1
Mediterranean 2
Mediterranean 3
Mediterranean 1
Mediterranean 2
Mediterranean 3
Population
RYGG
RYGG
RYGG
HAMM
HAMM
HAMM
INNF
INNF
INNF
RODA
RODA
RODA
SPE
SPE
SPE
POB
POB
POB
MUR
MUR
MUR
VDM
VDM
VDM
Latitude
59.38
59.38
59.38
59.78
59.78
59.78
62.5
62.5
62.5
62.8
62.8
62.8
41.76
41.76
41.76
41.35
41.35
41.35
41.34
41.34
41.34
42.03
42.03
42.03
Survival after
freezing (%)
50
50
53
47
49
53
96
96
98
71
77
80
3
5
5
7
16
17
36
37
44
40
49
53
Transcribed Image Text:Source of Seeds Trial # 1 Scandinavia Scandinavia 2 Scandinavia 3 Scandinavia 1 Scandinavia 2 Scandinavia 3 Scandinavia 1 Scandinavia 2 Scandinavia 3 Scandinavia 1 Scandinavia 2 Scandinavia 3 Mediterranean 1 Mediterranean 2 Mediterranean 3 Mediterranean 1 Mediterranean 2 Mediterranean 3 Mediterranean 1 Mediterranean 2 Mediterranean 3 Mediterranean 1 Mediterranean 2 Mediterranean 3 Population RYGG RYGG RYGG HAMM HAMM HAMM INNF INNF INNF RODA RODA RODA SPE SPE SPE POB POB POB MUR MUR MUR VDM VDM VDM Latitude 59.38 59.38 59.38 59.78 59.78 59.78 62.5 62.5 62.5 62.8 62.8 62.8 41.76 41.76 41.76 41.35 41.35 41.35 41.34 41.34 41.34 42.03 42.03 42.03 Survival after freezing (%) 50 50 53 47 49 53 96 96 98 71 77 80 3 5 5 7 16 17 36 37 44 40 49 53
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