DNA: How PCR works and why it's useful?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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DNA: How PCR works and why it's useful?
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Step 1
PCR is dependent on the ability of DNA polymerase to generate a new strand of DNA that is complementary to the provided template strand. Since DNA polymerase could only add nucleotides to a preexisting 3'-OH group, it requires a primer to which it may add the initial nucleotide. This requirement allows the researcher to designate a specific portion of the template sequence that he or she wishes to amplify. The specified sequence will be copied billions of times by the conclusion of the PCR process.
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