Answered: Discuss the difference(s) between the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Discuss the difference(s) between the separation of FAME on polar capillary column and non-polar capillary column.

Transcribed Image Text:Preparation of Fatty Acid Methyl Esters (Fames) and Determination of Fatty Acid Profile of Oils by Gas Chromatography (GC) Information about fatty acid profile on food is important for nutrition labeling, which involves the measurement of not only total fat but also saturated, unsaturated, and monounsaturated fat. Gas chromatography is an ideal instrument to determine (qualitatively and quantitatively) fatty acid profile or fatty acid composition of a food product. This usually involves extracting the lipids and analyzing them using capillary gas chromatography. Before such analysis, triacylglycerols and phospholipids are saponified and the fatty acids liberated are esterified to form fatty acid methyl esters (FAMES) so that the volatility is increased. The sample preparation for FAMES determination will be used in this experiment is the boron trifluoride (BF:) method. In the BF; method, lipids are saponified, and fatty acids are liberated and esterified in the presence of a BF3 catalyst for further analysis. This method is applicable to common animal and vegetable oils and fats, and fatty acids. Lipids that cannot be saponified are not derivatized and, if present in large amount, may interfere with subsequent analysis. This method is not suitable for preparation of methyl esters of fatty acids containing large amounts of epoxy, hydroperoxy, aldehyde, ketone, cyclopropyl, and cyclopentyl groups, and conjugated polyunsaturated and acetylenic compounds because of partial or complete destruction of these groups.

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