Discuss the appearance of cholesterol crystals under the microscope.
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Discuss the appearance of cholesterol crystals under the microscope.
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- Give information about the formation of acrylamide.Select lipid samples under triacylglycerol (one simple and one mixed), phospholipid, and cholesterol-derived metabolite. Show the structure and briefly describe the function or significance of each.Identify the structures shown as glycerophospholipids or sphingolipids.
- Name at least three test reactions for lipids. Briefly explain the the principle of each test and describe its positive result.Illustrate with equation the reaction of glucose, galactose, maltose & lactose with osazone test. Sketch the crystals formed for each of the following sugars examined under the microscope.What microscopic methods would be suitable for studying (a) the morphology of crystalline polymers, (b) surface oxidation in a hydrocarbon polymer, (c) the structure of the beads used in a GPC column, (d) suspected gel particles in branched polyethylene (e) How may x-ray diffraction data be used to estimate the sizes of polymer crystallites?
- determine the class of lipid shown and identify whether if it is saponifiable or nonsaponifiableExplain how do you prepare a 25-mL solution of 1 mg/mL cholesterol stock?a. Construct a typical wax derived from palmitic acid. No sticks are acceptable. CH2 groups can be bunched together. b. Construct a chiral triglyceride, using appropriate format. Again, no sticks. CH2 groups can be combined.
- Will salt crystals dissolve in pure methanol or pure water?A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.Define iodide pump.