Determine the Michaelis constant and the maximum velocity of the reaction. Using direct linear plot

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
icon
Related questions
Question

Determine the Michaelis constant and the maximum velocity of the reaction. Using direct linear plot

8.14 Isocitrate lyase catalyzes the following reaction:
isocitrate ion → glyoxylate ion + succinate ion
The rate, v, of the reaction was measured when various concentrations
of isocitrate ion were present, and the following results were obtained
at 25°C:
[isocitrate]/(µmol dm-³)
v/(pmol dm³s-1)
31.8
46.4
59.3
118.5
222.2
70.0
97.2
116.7
159.2
194.5
Determine the Michaelis constant and the maximum velocity of the
reaction.
Transcribed Image Text:8.14 Isocitrate lyase catalyzes the following reaction: isocitrate ion → glyoxylate ion + succinate ion The rate, v, of the reaction was measured when various concentrations of isocitrate ion were present, and the following results were obtained at 25°C: [isocitrate]/(µmol dm-³) v/(pmol dm³s-1) 31.8 46.4 59.3 118.5 222.2 70.0 97.2 116.7 159.2 194.5 Determine the Michaelis constant and the maximum velocity of the reaction.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 5 images

Blurred answer
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The