Determine the following for positive staining. (a) dye, (b) charge of the chromophore/colored ion, (c) charge of the target cellular structure, (d) applications/target cell structure 2.Determine the following for negative staining. (a) dye, (b) charge of the chromophore/colored ion, (c) charge of the target cellular structure, (d) applications/target cell structure
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1. Determine the following for positive staining. (a) dye, (b) charge of the chromophore/colored ion, (c) charge of the target cellular structure, (d) applications/target cell structure
2.Determine the following for negative staining. (a) dye, (b) charge of the chromophore/colored ion, (c) charge of the target cellular structure, (d) applications/target cell structure
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- 5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a small region of the cell membrane with an intense laser light, and determine the time it takes for the bleached spot to recover fluorescent signal (see figure below). (a) (b) Bleach Laser bleaching of fluorescent marker Fluorescence intensity recovery Recovery Time How would the recovery time of your protein change if the membrane contained a higher concentration of unsaturated fatty acids? Why? How would the recovery time of your protein change if you conducted the experiment at a lower temperature? Why? How would the recovery time of your protein change if it were anchored to the membrane skeleton? Why?Which of the following is/are true about fluorescentmicroscopy?(a) Fluorescent microscopes use an infrared light source(b) Fluorochromes are sometimes necessary to visualizecellular structures or cells(c) Antibodies can be “tagged” with fluorescent mole-cules to help visualize and prove the presence of theircorresponding antigen or foreign substance, such as amicrobe in a blood sample(d) a, b, and c(e) b and c(f) None of the aboveUV light has the greatest direct effect on protein structure within a cell, causing crosslinking of proteins. O 1) True 2) False
- ASAPWhich of the following statements about electron microscopy are true? a) Most existing protein structures have been resolved using electron microscopy b) Electron microscopy is not used in structural biology as it can not give as high a resolution as X-ray crystallography and NMR c) The smaller a protein, the easier it is to solve its structure with electron microscopy d) By taking pictures of the same protein frozen in ice thousands of times and then adding them together, you get a high-resolution image of the protein e) The first protein structure with true atomic resolution was solved using electron microscopy last yearIn your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average value
- Discussion Give the main differences between the types of microscopes used to diagnose surfaces?) What is the appropriate device to diagnose each of the following and why? O A- The aluminum plate is coated with a nano-solution. b- A colloidal sample containing nano-solutions. C- Study of the microstructure of the surface of a sample of iron.' Is it possible to use an optical microscope to study painted metal) surfaces? And why?8.1f you collect a swab of cells from the inside of your mouth and perform the Gram stain technique, what would be the result? Would your epithelial cells be Gram positive or Gram negative? Explain the result based upon the cell structure1. Typical microorganisms are on the scale of 1 micrometers. The typical human eye can see things as small as 0.1 millimeter unaided. With a standard microscope, you can see things on a scale of 100 nanometers. (a) Can you see microorganisms with your eye? With a microscope? Justify your an- swer by writing everything in scientific notation. (b) How many times magnified does a standard microscope make something look, as compared to a bare eye?
- 3. Hydrophobic signal molecules typically ... a) bind to receptor proteins in the cytosol. b) bind to plasma membrane receptors then move inside the cell. c) activate pathways that drive gene transcription. d) a and c e) a, b and cPlease provide the mechanisms of the active transport of nano carriers used for crossing the blood brain barriers with detailed explanation and requirement needed to cross the blood brain barriers (what types of structures of the compounds, sizes). I want clear handwritten solution only....i will up voteIn the space provided below, give me the word(s) that would correctly complete each sentence: A) The equation "d = A/ (2 NA)" describes the of a lens system B) In the flagella stain, the mordant makes bacterial flagella easier to see by making them