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describe the 3 processes that use moist heat methods specifying time and temperature for each:
pasteurization
autoclaving
tyndallization
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- Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.What are the primary uses of the following preparations of culture media? Agar regular slant Agar special slant Agar deep Broth Agar plateAs the temperature of pasteurization increases, the time of treatment decreases. As the temperature of pasteurization increases, the time of treatment decreases. True False
- Define a pure culture.Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
- You have a broth medium that you would like to turn into a solid medium in order to do streak plates. What could you add to your media to allow it to solidify? Paragraph В I UChoose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage: a) Drying b) Mineral oil overlay c) Freezing with liquid nitrogen d) Lyophilization e) Periodic transfer to fresh mediumDistinguish batch culture and continuous culture
- Aside from application of heat, name other methods of sterilization and give examples of materials sterilized in each methodWhy is dilution a necessary part of pure culture preparation?Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…