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Describe how you would dilute a bacterial culture by 10-7
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- Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.
- How would you prepare a 10-7dilution from a culture? Each of your dilution tubes cannot hold more than 1mL.You were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)How long does it take for a bacterial culture with a generation time of 12 minutes to increase from 400 cells up to 1600 cells/mL?
- Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoDescribe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.Inoculate one colony from LB plate into 5 mL LB liquid medium. Shake at 37 °C overnight. Why inoculate from just a single colony?
- You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volumeWhere are you most likely to be using acids and alkalis for bacterial control?How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.