Answer both plz. Question 3What are the typical features that enable efficient cloning in a prokaryotic cell? Select all correctanswers.O Selectable markerO Blue/white selectionO Amino acid synthesisO PolylinkerO Origin of replicationQuestion 4In a cloning experiment in E. coli, DNA ligase frequently causes "self-ligation" of the digestedplasmid, resulting in an empty vector.O TrueO False

Hi, Thanks For Your Question. Q. 3 Answer : Correct Options Are ; Origin Of Replication And…

D Question 3 What are the typical features that enable efficient cloning in a prokaryotic cell? Select all correct answers. Selectable marker Blue/white selection Amino acid synthesis Polylinker Origin of replication D Question 4 In a cloning experiment in E. coli, DNA ligase frequently causes "self-ligation" of the digested plasmid, resulting in an empty vector. True False

D Question 3 What are the typical features that enable efficient cloning in a prokaryotic cell? Select all correct answers. Selectable marker Blue/white selection Amino acid synthesis Polylinker Origin of replication D Question 4 In a cloning experiment in E. coli, DNA ligase frequently causes "self-ligation" of the digested plasmid, resulting in an empty vector. True False

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 9SQ

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QUESTION 14 What if you designed a CRISPR/cas9 assay for a gene knockout, but there's no PAM sequence immediately downstream of your target site. What happens? O It's fine because you put the PAM sequence in your guide RNA O PAM sequences only apply to real viral infections, not CRISPR assays. O The Cas9 nuclease won't cut at the target site O The guide RNA won't be complementary to the target O They'll be more off target effects but it'll still work.
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Alternative splicing Template strand S F yIGUide1,mq00:c-00: Replisome Transforming principle Origin of replication (or)eleb al msxS ain Coding strand Transcription factors Leading strand Single nucleotide polymorphism Okazaki fragment Telomerase M Nucleoside RNA Polymerase I RNA Polymerase II RNA Polymerase IIIon & of qu 9ven UoY Insertion mutagenesis Spliceosome Transcription Unit SNP Reverse transcriptase 1 Seminal work by Oswald Avery and colleagues demonstrated that DNA is what Frederick Griffiths called this etniog OS dotsM bioW 1-2kb of newly synthesized DNA strands are called this ainiog PS Assembly of the replisome is an orderly process that begins at these precise sites Snoiteeu 4 Transcribes ribosomal RNA genes in eukaryotes 5. A large nucleoprotein complex that coordinates activity at the replication fork Single base pair differences between homologous genomic regions isolated from different members of a population Complex of proteins and snRNAs catalyzing the removal of…
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Alu 1 EcoRI ori pKY Bgl I Tet: Tetracycline resistance gene A. What would be the donor organism to be used in this cloning? B. How would you make sure you only get your gene of interest (EGF) from donor but nothing else? C. How do you put EGF gene into pKY plasmid? What is the best RE to use when inserting the gene into pKY plasmid? What happens if you use the enzyme Bgl I to insert the gene of interest? How does it affect your cloning result? D. How do you make sure you only grow E.coli that have the gene of interest?
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Homologous Recombination, Heteroduplex DNA, and Mismatch Repair Homologous recombination in E. coli leads to the formation of regions of heteroduplex DNA. By definition, such regions contain mismatched bases. Why doesnâ€™t the mismatch repair system of E. coli eliminate these mismatches?
The ends of the linear chromosomes are maintained by helicase primase DNA pol telomerase